Expression of cytokine genes in Aotus monkeys immunized with synthetic and recombinant Plasmodium vivax and P. falciparum antigens.
Expression of cytokine genes in Aotus monkeys immunized with synthetic and recombinant Plasmodium vivax and P. falciparum antigens.
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用合成和重组间日疟原虫和恶性疟原虫抗原免疫的白猴中细胞因子基因的表达。
DOI：
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发表时间：
1998
影响因子：
--
通讯作者：
M. James
中科院分区：
文献类型：
--
作者：
S. Duque;S. Montenegro;M. Arevalo;A. Praba;F. Villinger;S. Herrera;M. James
人类宿主对疟疾的保护性免疫中的细胞因子应答尚未完全阐明。似乎没有数据存在的非人灵长类动物模型免疫疟疾抗原的细胞因子模式。用逆转录酶PCR法检测了9只猕猴外周血单个核细胞（PBMC）中10种细胞因子、粘附分子ICAM-1和诱导型一氧化氮合酶（iNOS）mRNA的表达。五只猴子已经用间日疟原虫环子孢子蛋白的多抗原肽（MAP）免疫，两只猴子用恶性疟原虫裂殖子表面蛋白-1（MSP-1）的构建体免疫。另外两只猴子作为非免疫对照。PBMC在用植物血凝素促分裂原、MAP和MSP-1抗原刺激后培养24小时。在对MAP的反应中观察到白细胞介素-6（IL-6）、IL-10、IL-12、肿瘤坏死因子-α（TNF-α）、TNF-β和iNOS的表达升高。用恶性疟原虫MSP r190 L或合成190 L肽免疫的猴主要表达1型细胞因子（IL-1 β、IL-12、干扰素-γ、TNF-α、TNF-β），其特征在于脾细胞介导的活性，具有巨噬细胞活化和一氧化氮产生。
Cytokine responses in human host-protective immunity to malaria have yet to be completely elucidated. No data appear to exist on the cytokine patterns in non-human primate models immunized with malarial antigens. Expression of mRNA transcripts of 10 cytokines, the adhesion molecule ICAM-1 and inducible nitric oxide synthase (iNOS) in peripheral-blood mononuclear cells (PBMC) from nine Aotus monkeys was analysed by reverse-transcriptase PCR. Five of the monkeys had been immunized with multiple-antigen peptides (MAP) of the Plasmodium vivax circumsporozoite protein and two with constructs of the P. falciparum merozoite surface protein-1 (MSP-1). The other two monkeys served as non-immunized controls. PBMC were cultured for 24 h after stimulation with phytohaemagglutinin mitogen, MAP and MSP-1 antigens. Elevated expression of interleukin-6 (IL-6), IL-10, IL-12, tumour necrosis factor-alpha (TNF-alpha), TNF-beta and iNOS was seen in response to the MAP. Monkeys immunized with either P. falciparum MSP r190L or synthetic 190L peptides expressed predominantly the type-1 cytokines (IL-1 beta, IL-12, interferon-gamma, TNF-alpha, TNF-beta) characteristic of splenic, cell-mediated activity with macrophage activation and nitric oxide production.
定义对病原体的保护性反应：麻风病灶中的细胞因子谱。
DOI：
10.1126/science.254.5029.277
发表时间：
1991
期刊：
Science (New York, N.Y.)
影响因子：
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作者：
Yamamura,M;Uyemura,K;Deans,RJ;Weinberg,K;Rea,TH;Bloom,BR;Modlin,RL
通讯作者：
Modlin,RL
非人灵长类动物外周血单核细胞合成和分泌的细胞因子的定性和定量研究。
影响因子：
3.8
作者：
Villinger,F;Hunt,D;Mayne,A;Vuchetich,M;Findley,H;Ansari,AA
通讯作者：
Ansari,AA
猫头鹰猴 (Aotus trivirgatus) 的 T 辅助细胞 1 型和 T 辅助细胞 2 型淋巴因子基因的逆转录酶-聚合酶链式反应扩增和部分序列。
DOI：
10.4269/ajtmh.1997.56.351
发表时间：
1997
期刊：
The American journal of tropical medicine and hygiene
影响因子：
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作者：
Bost,KL;HellnerJr,CF;Holton,RH;Ratterree,MS;Clements,JD;Krogstad,DJ;Kincy-Cain,T
通讯作者：
Kincy-Cain,T