Rapid Bacterial Motility Monitoring Using Inexpensive 3D-Printed OpenFlexure Microscopy Allows Microfluidic Antibiotic Susceptibility Testing.
Rapid Bacterial Motility Monitoring Using Inexpensive 3D-Printed OpenFlexure Microscopy Allows Microfluidic Antibiotic Susceptibility Testing.
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DOI：
10.3390/mi13111974
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发表时间：
2022-11-14
期刊：
影响因子：
3.4
通讯作者：
Edwards AD
中科院分区：
文献类型：
--
作者：
Diep TT;Needs SH;Bizley S;Edwards AD
抗生素敏感性测试对于解决抗生素耐药性的出现和传播至关重要。廉价的数字CMOS相机可以使用3D打印的x-y-z载物台转换为便携式数字显微镜。显微镜检查细菌运动性可以快速检测微生物对抗生素的反应，以确定敏感性。在这里，我们提出了一种新的简单的微装置微型显微镜细胞测量系统的多重抗生素敏感性测试。微型装置使用含有十个平行的0.2 mm直径微毛细管的熔融挤出塑料膜条制成。在Mueller-Hinton琼脂（0.4%）中制备了两种不同的抗生素，头孢他啶和庆大霉素，以生产用于简单样品添加的抗生素加载微装置。选择该组合以紧密匹配抗生素敏感性测试和运动性测试的当前标准方法。使用低浓度琼脂可观察到运动细菌进入毛细血管时对抗生素暴露的反应。该设备适合使用Raspberry Pi计算机和v2相机的OpenFlexure 3D打印数字显微镜，避免了昂贵的实验室显微镜的需要。这种便宜的便携式数字显微镜平台具有足够的放大倍数来检测运动细菌，但又有足够宽的视野来监测细菌进入载药微毛细管时的行为。图像质量足以检测不同浓度的抗生素如何抑制细菌运动。我们的结论是，基于Raspberry Pi的3D打印显微镜与一次性微流体测试条相结合，可以快速，易于使用的细菌运动检测，具有帮助检测抗生素耐药性的潜力。
Antibiotic susceptibility testing is vital to tackle the emergence and spread of antimicrobial resistance. Inexpensive digital CMOS cameras can be converted into portable digital microscopes using 3D printed x-y-z stages. Microscopic examination of bacterial motility can rapidly detect the response of microbes to antibiotics to determine susceptibility. Here, we present a new simple microdevice-miniature microscope cell measurement system for multiplexed antibiotic susceptibility testing. The microdevice is made using melt-extruded plastic film strips containing ten parallel 0.2 mm diameter microcapillaries. Two different antibiotics, ceftazidime and gentamicin, were prepared in Mueller-Hinton agar (0.4%) to produce an antibiotic-loaded microdevice for simple sample addition. This combination was selected to closely match current standard methods for both antibiotic susceptibility testing and motility testing. Use of low agar concentration permits observation of motile bacteria responding to antibiotic exposure as they enter capillaries. This device fits onto the OpenFlexure 3D-printed digital microscope using a Raspberry Pi computer and v2 camera, avoiding need for expensive laboratory microscopes. This inexpensive and portable digital microscope platform had sufficient magnification to detect motile bacteria, yet wide enough field of view to monitor bacteria behavior as they entered antibiotic-loaded microcapillaries. The image quality was sufficient to detect how bacterial motility was inhibited by different concentrations of antibiotic. We conclude that a 3D-printed Raspberry Pi-based microscope combined with disposable microfluidic test strips permit rapid, easy-to-use bacterial motility detection, with potential for aiding detection of antibiotic resistance.
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影响因子：
3.7
作者：
Gurdita A;Vovko H;Ungrin M
通讯作者：
Ungrin M
影响因子：
9.8
作者：
Baden T;Chagas AM;Gage GJ;Marzullo TC;Prieto-Godino LL;Euler T
通讯作者：
Euler T
Pispy：一个负担得起的，易于访问且灵活的成像平台，用于自动观察有机生物学和行为。
影响因子：
3.7
作者：
Morris BI;Kittredge MJ;Casey B;Meng O;Chagas AM;Lamparter M;Thul T;Pask GM
通讯作者：
Pask GM
影响因子：
6.1
作者：
Pitruzzello, Giampaolo;Thorpe, Stephen;Krauss, Thomas F.
通讯作者：
Krauss, Thomas F.
影响因子：
3.4
作者：
Collins, Joel Thomas;Knapper, Joe;Bowman, Richard
通讯作者：
Bowman, Richard