PiSpy: An affordable, accessible, and flexible imaging platform for the automated observation of organismal biology and behavior.

PiSpy: An affordable, accessible, and flexible imaging platform for the automated observation of organismal biology and behavior.
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Pispy:一个负担得起的,易于访问且灵活的成像平台,用于自动观察有机生物学和行为。

DOI:
10.1371/journal.pone.0276652
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发表时间:
2022
期刊:
影响因子:
3.7
通讯作者:
Pask GM
Pask GM
中科院分区:
综合性期刊3区
文献类型:
--
作者:
Morris BI;Kittredge MJ;Casey B;Meng O;Chagas AM;Lamparter M;Thul T;Pask GM

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从对有机体生长、发育和行为的直接观察中可以获得大量的理解。然而,直接观察可能会耗费时间,并通过无意的刺激影响生物体。此外,视频捕捉设备通常非常昂贵,难以根据个人的特定需求进行修改,并且可能带有不必要的功能。在这里,我们描述了PiSpy,一个低成本的自动化视频采集平台,它使用树莓派计算机和相机在指定的时间间隔或外部触发时记录视频或图像。所有的设置和控制,如可编程的光循环,可访问的用户没有编程经验,通过一个易于使用的图形用户界面。重要的是,使用激光切割和3d打印组件,整个PiSpy系统的组装成本不到100美元。我们展示了PiSpy在一系列模型和非模型生物中的广泛应用和灵活性。设计、说明和代码可以通过在线存储库访问,在该存储库中,PiSpy用户的全球社区也可以贡献他们自己独特的定制,并帮助发展开源研究解决方案社区。
A great deal of understanding can be gleaned from direct observation of organismal growth, development, and behavior. However, direct observation can be time consuming and influence the organism through unintentional stimuli. Additionally, video capturing equipment can often be prohibitively expensive, difficult to modify to one’s specific needs, and may come with unnecessary features. Here, we describe PiSpy, a low-cost, automated video acquisition platform that uses a Raspberry Pi computer and camera to record video or images at specified time intervals or when externally triggered. All settings and controls, such as programmable light cycling, are accessible to users with no programming experience through an easy-to-use graphical user interface. Importantly, the entire PiSpy system can be assembled for less than $100 using laser-cut and 3D-printed components. We demonstrate the broad applications and flexibility of PiSpy across a range of model and non-model organisms. Designs, instructions, and code can be accessed through an online repository, where a global community of PiSpy users can also contribute their own unique customizations and help grow the community of open-source research solutions.
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