Control of Western Corn Rootworm (Diabrotica virgifera virgifera) Reproduction through Plant-Mediated RNA Interference.
Control of Western Corn Rootworm (Diabrotica virgifera virgifera) Reproduction through Plant-Mediated RNA Interference.
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DOI：
10.1038/s41598-017-12638-3
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发表时间：
2017-10-03
影响因子：
4.6
通讯作者：
Wu G
中科院分区：
文献类型：
--
作者：
Niu X;Kassa A;Hu X;Robeson J;McMahon M;Richtman NM;Steimel JP;Kernodle BM;Crane VC;Sandahl G;Ritland JL;Presnail JK;Lu AL;Wu G
转基因玉米中的RNA干扰（RNAi）最近已经成为西方玉米根虫（玉米根萤叶甲）控制的替代作用模式，其可以与基于蛋白质的根虫控制选项组合以改善根部保护和抗性管理。目前，基于转基因RNAi的控制集中在抑制当沉默时导致幼虫死亡的基因。我们研究了通过RNAi通过靶向两个生殖基因dvvgr和dvbol来控制西方玉米根虫生殖，目的是降低昆虫繁殖力作为害虫管理的新工具。结果表明，成虫以及幼虫暴露于人工饲料中的dvvgr或dvbol dsRNA，导致繁殖力降低。此外，从在表达dvbol dsRNA的转基因玉米根上取食的幼虫出现的西方玉米根虫甲虫也显示出显著的繁殖力降低。这是第一个通过植物介导的RNAi降低昆虫生殖适合度的报道，证明了生殖RNAi作为西方玉米根虫管理工具的可行性。
RNA interference (RNAi) in transgenic maize has recently emerged as an alternative mode of action for western corn rootworm (Diabrotica virgifera virgifera) control which can be combined with protein-based rootworm control options for improved root protection and resistance management. Currently, transgenic RNAi-based control has focused on suppression of genes that when silenced lead to larval mortality. We investigated control of western corn rootworm reproduction through RNAi by targeting two reproductive genes, dvvgr and dvbol, with the goal of reducing insect fecundity as a new tool for pest management. The results demonstrated that exposure of adult beetles, as well as larvae to dvvgr or dvbol dsRNA in artificial diet, caused reduction of fecundity. Furthermore, western corn rootworm beetles that emerged from larval feeding on transgenic maize roots expressing dvbol dsRNA also showed significant fecundity reduction. This is the first report of reduction of insect reproductive fitness through plant-mediated RNAi, demonstrating the feasibility of reproductive RNAi as a management tool for western corn rootworm.
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DOI：
10.1261/rna.048116.114
发表时间：
2015-05
期刊：
RNA (New York, N.Y.)
影响因子：
--
作者：
Ivashuta S;Zhang Y;Wiggins BE;Ramaseshadri P;Segers GC;Johnson S;Meyer SE;Kerstetter RA;McNulty BC;Bolognesi R;Heck GR
通讯作者：
Heck GR
影响因子：
4.6
作者：
Jakka, Siva R. K.;Shrestha, Ram B.;Gassmann, Aaron J.
通讯作者：
Gassmann, Aaron J.
影响因子：
3.7
作者：
Gassmann AJ;Petzold-Maxwell JL;Keweshan RS;Dunbar MW
通讯作者：
Dunbar MW
影响因子：
6.2
作者：
Cho, Myeong-Je;Wu, Emily;Zhao, Zuo-Yu
通讯作者：
Zhao, Zuo-Yu
影响因子：
1.9
作者：
Li, H.;Khajuria, C.;Narva, K. E.
通讯作者：
Narva, K. E.