A PDMS/paper/glass hybrid microfluidic biochip integrated with aptamer-functionalized graphene oxide nano-biosensors for one-step multiplexed pathogen detection.
A PDMS/paper/glass hybrid microfluidic biochip integrated with aptamer-functionalized graphene oxide nano-biosensors for one-step multiplexed pathogen detection.
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DOI：
10.1039/c3lc50654a
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发表时间：
2013-10-07
期刊：
影响因子：
6.1
通讯作者：
Ye BC
中科院分区：
文献类型：
--
作者：
Zuo P;Li X;Dominguez DC;Ye BC
传染性病原体经常在世界各地引起严重的公共卫生问题。对用于多重病原体检测的简单、快速和灵敏的方法的需求越来越大。本文开发了一种聚二甲基硅氧烷(PDMS)/纸/玻璃混合微流控系统，与适体功能化的氧化石墨烯(GO)纳米生物传感器集成在一起，用于简单、一步、多重病原体检测。该混合微流控系统中使用的纸基促进了适体生物传感器在微流控芯片上的集成，并避免了PDMS或纯玻璃微流控系统中复杂的表面处理和适体探针的固定。以嗜酸乳杆菌为细菌模型，建立了检测下限为11.0cfu m L−1的微流控检测平台，并成功地将该方法扩展到金黄色葡萄球菌和肠炎沙门氏菌两种感染性病原体的同时检测。该方法简单、快速。在即用型微流控设备中，只需10分钟左右即可在生物芯片上完成病原体分析。此外，这种微流控设备在快速检测各种不同的其他细菌和病毒病原体方面具有巨大的潜力。
Infectious pathogens often cause serious public health concerns throughout the world. There is an increasing demand for simple, rapid and sensitive approaches for multiplexed pathogen detection. In this paper we have developed a polydimethylsiloxane (PDMS)/paper/glass hybrid microfluidic system integrated with aptamer-functionalized graphene oxide (GO) nano-biosensors for simple, one-step, multiplexed pathogen detection. The paper substrate used in this hybrid microfluidic system facilitated the integration of aptamer biosensors on the microfluidic biochip, and avoided complicated surface treatment and aptamer probe immobilization in a PDMS or glass-only microfluidic system. Lactobacillus acidophilus was used as a bacterium model to develop the microfluidic platform with a detection limit of 11.0 cfu mL−1. We have also successfully extended this method to the simultaneous detection of two infectious pathogens - Staphylococcus aureus and Salmonella enterica. This method is simple and fast. The one-step ‘turn on’ pathogen assay in a ready-to-use microfluidic device only takes ~10 min to complete on the biochip. Furthermore, this microfluidic device has great potential in rapid detection of a wide variety of different other bacterial and viral pathogens.
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