Meiotic nuclear pore complex remodeling provides key insights into nuclear basket organization.
Meiotic nuclear pore complex remodeling provides key insights into nuclear basket organization.
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减数分裂核孔复合物的重塑为核篮组织提供了关键的见解。
DOI：
10.1083/jcb.202204039
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发表时间：
2023-02-06
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影响因子：
--
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中科院分区：
文献类型：
--
作者：
King和Wettstein等人揭示了核孔复合物在芽殖酵母减数分裂期间经历两种不同的重塑事件：部分和完全核篮脱离。在解剖这些事件的调节，这项研究提供了深入了解核篮组织。核孔复合物（NPC）是介导核区室化的大型蛋白质组装体。NPC在后生动物和一些真菌的有丝分裂过程中经历大规模的结构重排。然而，我们对有丝分裂后NPC重塑的理解仍然有限。使用延时荧光显微镜，我们发现，NPC在芽殖酵母减数分裂过程中经历了两个机械上可分离的重塑事件，其中部分或全部核篮在减数分裂I和II期间分别与NPC核心短暂解离。对于Nup 60和Nup 2观察到的减数分裂I脱离是由Nup 60在其与Y复合物的界面处的波罗激酶介导的磷酸化驱动的。Nup 60中的脂质结合两亲性螺旋促进Nup 60-Nup 2随后重新附着到NPC核心。阻止Nup 60-Nup 2再附着会导致配子中整个核篮的错误组织。引人注目的是，减数分裂核篮重塑也发生在远亲裂殖酵母，裂殖酵母。我们的研究揭示了NPC可塑性的保守和发育编程方面，为核篮组织提供了关键的机械见解。
King and Wettstein et al. reveal that nuclear pore complexes undergo two distinct remodeling events during budding yeast meiosis: partial and full nuclear basket detachment. In dissecting the regulation of these events, this study provides insights into nuclear basket organization. Nuclear pore complexes (NPCs) are large proteinaceous assemblies that mediate nuclear compartmentalization. NPCs undergo large-scale structural rearrangements during mitosis in metazoans and some fungi. However, our understanding of NPC remodeling beyond mitosis remains limited. Using time-lapse fluorescence microscopy, we discovered that NPCs undergo two mechanistically separable remodeling events during budding yeast meiosis in which parts or all of the nuclear basket transiently dissociate from the NPC core during meiosis I and II, respectively. Meiosis I detachment, observed for Nup60 and Nup2, is driven by Polo kinase-mediated phosphorylation of Nup60 at its interface with the Y-complex. Subsequent reattachment of Nup60-Nup2 to the NPC core is facilitated by a lipid-binding amphipathic helix in Nup60. Preventing Nup60-Nup2 reattachment causes misorganization of the entire nuclear basket in gametes. Strikingly, meiotic nuclear basket remodeling also occurs in the distantly related fission yeast, Schizosaccharomyces pombe. Our study reveals a conserved and developmentally programmed aspect of NPC plasticity, providing key mechanistic insights into the nuclear basket organization.
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影响因子：
64.5
作者：
Cheng Z;Otto GM;Powers EN;Keskin A;Mertins P;Carr SA;Jovanovic M;Brar GA
通讯作者：
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7.8
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16.6
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Panse VG
影响因子：
9.2
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Charles, JF;Jespersen, SL;Morgan, DO
通讯作者：
Morgan, DO