Closed mitosis requires local disassembly of the nuclear envelope.
Closed mitosis requires local disassembly of the nuclear envelope.
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DOI：
10.1038/s41586-020-2648-3
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发表时间：
2020-09
期刊：
影响因子：
64.8
通讯作者：
Baum B
中科院分区：
文献类型：
--
作者：
Dey G;Culley S;Curran S;Schmidt U;Henriques R;Kukulski W;Baum B
在有丝分裂结束时，真核细胞必须将其复制的基因组的两个副本分离到两个新的核隔室中。要做到这一点，要么首先拆解核膜，然后在所谓的“开放有丝分裂”中重新组装核膜，要么重塑一个完整的细胞核，然后在“闭合有丝分裂”中分裂成两个。虽然有丝分裂在各种各样的真核生物中已经被研究了一个多世纪，但在闭合有丝分裂结束时，核膜的双膜如何在不影响核室的不通透性的情况下分裂成两部分，这一点尚不清楚。在裂解酵母中研究这一问题时，我们使用遗传学、活细胞成像和电子断层扫描技术，证明核分裂是通过连接分离子核的狭窄桥内的核膜(NE)局部分解来实现的。在这样做的过程中，我们发现了一种新的内部NE定位的蛋白质Les1，它将局部NE的破坏过程限制在桥的中段，以防止物质从子核泄漏。闭合有丝分裂中局部NEB的机制与开放有丝分裂中NEB的机制非常相似，揭示了不同真核生物中核重塑机制出人意料的深度保守。
At the end of mitosis, eukaryotic cells must segregate both copies of their replicated genome into two new nuclear compartments. They do this either by first dismantling and later reassembling the nuclear envelope in a so called “open mitosis”, or by reshaping an intact nucleus and then dividing into two in a “closed mitosis”. While mitosis has been studied in a wide variety of eukaryotes for over a century, it is not known how the double membrane of the nuclear envelope is split into two at the end of a closed mitosis without compromising the impermeability of the nuclear compartment. In studying this problem in the fission yeast Schizosaccharomyces pombe, a classical model for closed mitosis, we use genetics, live cell imaging and electron tomography to show that nuclear fission is achieved via local disassembly of the nuclear envelope (NE) within the narrow bridge that links segregating daughter nuclei. In doing so, we identify a novel inner NE-localised protein Les1 that restricts the process of local NE breakdown (local NEB) to the bridge midzone to prevent the leakage of material from daughter nuclei. The mechanics of local NEB in a closed mitosis closely mirror those of NEB in open mitosis, revealing an unexpectedly deep conservation of nuclear remodelling mechanisms across diverse eukaryotes.
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影响因子：
9.5
作者：
Katoh K;Rozewicki J;Yamada KD
通讯作者：
Yamada KD
影响因子：
64.5
作者：
Frost A;Elgort MG;Brandman O;Ives C;Collins SR;Miller-Vedam L;Weibezahn J;Hein MY;Poser I;Mann M;Hyman AA;Weissman JS
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作者：
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作者：
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通讯作者：
Ullman, Katharine S.
DOI：
10.1093/bioinformatics/btp348
发表时间：
2009-08-01
期刊：
Bioinformatics (Oxford, England)
影响因子：
--
作者：
Capella-Gutiérrez S;Silla-Martínez JM;Gabaldón T
通讯作者：
Gabaldón T