Pervasive, Coordinated Protein-Level Changes Driven by Transcript Isoform Switching during Meiosis.
Pervasive, Coordinated Protein-Level Changes Driven by Transcript Isoform Switching during Meiosis.
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DOI：
10.1016/j.cell.2018.01.035
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发表时间：
2018-02-22
期刊：
影响因子：
64.5
通讯作者：
Brar GA
中科院分区：
文献类型：
--
作者：
Cheng Z;Otto GM;Powers EN;Keskin A;Mertins P;Carr SA;Jovanovic M;Brar GA
为了更好地了解基因调控机制，程序的发展过程中，我们进行了同步，全基因组测量的mRNA，翻译和蛋白质，通过减数分裂分化芽殖酵母。令人惊讶的是，我们观察到数百种mRNA的水平与其相应的蛋白质产物反相关。我们表明，而不是从典型形式的基因调控控制，至少380个这样的情况下，或超过8%的所有测量的基因的调节涉及时间调节转换之间的生产的一个典型的，可翻译的转录本和5′延伸的亚型，不能有效地翻译成蛋白质。通过这种通过自然发育程序调节蛋白质水平的普遍机制，单个转录因子可以协调激活和抑制不同基因组的蛋白质合成。这种区别不是基于mRNA是否被诱导，而是基于产生的转录物的类型。
To better understand the gene regulatory mechanisms that program developmental processes, we carried out simultaneous, genome-wide measurements of mRNA, translation and protein through meiotic differentiation in budding yeast. Surprisingly, we observed that the levels of several hundred mRNAs are anti-correlated with their corresponding protein products. We show that rather than arising from canonical forms of gene regulatory control, the regulation of at least 380 such cases—or over 8% of all measured genes—involves temporally regulated switching between production of a canonical, translatable transcript and a 5′ extended isoform that is not efficiently translated into protein. By this pervasive mechanism for the modulation of protein levels through a natural developmental program, a single transcription factor can coordinately activate and repress protein synthesis for distinct sets of genes. The distinction is not based on whether an mRNA is induced or not, but rather based on the type of transcript produced.
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影响因子：
48
作者：
Mertins, Philipp;Qiao, Jana W.;Patel, Jinal;Udeshi, Namrata D.;Clauser, Karl R.;Mani, D. R.;Burgess, Michael W.;Gillette, Michael A.;Jaffe, Jacob D.;Carr, Steven A.
通讯作者：
Carr, Steven A.
影响因子：
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影响因子：
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影响因子：
8.8
作者：
Kronja I;Yuan B;Eichhorn SW;Dzeyk K;Krijgsveld J;Bartel DP;Orr-Weaver TL
通讯作者：
Orr-Weaver TL
影响因子：
64.8
作者：
Martens, JA;Laprade, L;Winston, F
通讯作者：
Winston, F