Comparative Genomics and Description of Putative Virulence Factors of Melissococcus plutonius, the Causative Agent of European Foulbrood Disease in Honey Bees.
Comparative Genomics and Description of Putative Virulence Factors of Melissococcus plutonius, the Causative Agent of European Foulbrood Disease in Honey Bees.
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DOI：
10.3390/genes9080419
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发表时间：
2018-08-20
期刊：
影响因子：
3.5
通讯作者：
Poehlein A
中科院分区：
文献类型：
--
作者：
Djukic M;Erler S;Leimbach A;Grossar D;Charrière JD;Gauthier L;Hartken D;Dietrich S;Nacke H;Daniel R;Poehlein A
在欧洲，大约 84% 的栽培作物物种依赖昆虫授粉，主要是蜜蜂。 Apis mellifera（西方蜜蜂）是世界上最重要的商业授粉媒介。革兰氏阳性菌梅利索球菌是欧洲蜂巢腐臭病 (EFB) 的病原体，这是一种全球性蜜蜂巢病。为了检测假定的毒力因子，我们对 14 个 M. plutonius 菌株（包括两个参考分离株）的基因组进行了测序和分析。这些分离株在基因组大小或预测的蛋白质编码基因数量方面没有表现出高度多样性，范围分别为 2.021 至 2.101 Mbp 和 1589 至 1686。比较基因组学检测到可能在 EFB 发病机制中发挥作用并最终导致蜜蜂幼虫死亡的基因。这些包括细菌素、细菌细胞表面和宿主细胞粘附相关蛋白、肠球菌多糖抗原、ε毒素、蛋白水解酶和荚膜相关蛋白。使用自然感染的幼虫验证了三种假定毒力因子（内切-α-N-乙酰半乳糖胺酶、enhancin 和 epsilon 毒素）的体内表达。通过我们的菌株收集，我们首次表明无毒力和毒力典型菌株以及高毒力非典型菌株之间存在基因组差异，这可能导致 M. plutonius 的毒力。最后，我们还检测到每个基因组有大量保守的假基因（75 至 156 个），这表明进化宿主适应过程中基因组减少。
In Europe, approximately 84% of cultivated crop species depend on insect pollinators, mainly bees. Apis mellifera (the Western honey bee) is the most important commercial pollinator worldwide. The Gram-positive bacterium Melissococcus plutonius is the causative agent of European foulbrood (EFB), a global honey bee brood disease. In order to detect putative virulence factors, we sequenced and analyzed the genomes of 14 M. plutonius strains, including two reference isolates. The isolates do not show a high diversity in genome size or number of predicted protein-encoding genes, ranging from 2.021 to 2.101 Mbp and 1589 to 1686, respectively. Comparative genomics detected genes that might play a role in EFB pathogenesis and ultimately in the death of the honey bee larvae. These include bacteriocins, bacteria cell surface- and host cell adhesion-associated proteins, an enterococcal polysaccharide antigen, an epsilon toxin, proteolytic enzymes, and capsule-associated proteins. In vivo expression of three putative virulence factors (endo-alpha-N-acetylgalactosaminidase, enhancin and epsilon toxin) was verified using naturally infected larvae. With our strain collection, we show for the first time that genomic differences exist between non-virulent and virulent typical strains, as well as a highly virulent atypical strain, that may contribute to the virulence of M. plutonius. Finally, we also detected a high number of conserved pseudogenes (75 to 156) per genome, which indicates genomic reduction during evolutionary host adaptation.
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如何杀死蜜蜂幼虫：幼虫的基因组潜力和毒力机制。
影响因子：
3.7
作者：
Djukic M;Brzuszkiewicz E;Fünfhaus A;Voss J;Gollnow K;Poppinga L;Liesegang H;Garcia-Gonzalez E;Genersch E;Daniel R
通讯作者：
Daniel R
DOI：
10.1038/ismej.2014.20
发表时间：
2014-08
期刊：
The ISME journal
影响因子：
--
作者：
Budge GE;Shirley MD;Jones B;Quill E;Tomkies V;Feil EJ;Brown MA;Haynes EG
通讯作者：
Haynes EG
影响因子：
4.4
作者：
Connil, N;Le Breton, Y;Prévost, H
通讯作者：
Prévost, H
影响因子：
1.7
作者：
Bankevich, Anton;Nurk, Sergey;Pevzner, Pavel A.
通讯作者：
Pevzner, Pavel A.
影响因子：
4.4
作者：
Fang, Shangling;Wang, Li;Sun, Ming
通讯作者：
Sun, Ming