Multifarious Translational Regulation during Replicative Aging in Yeast.
Multifarious Translational Regulation during Replicative Aging in Yeast.
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DOI：
10.3390/jof8090938
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发表时间：
2022-09-05
期刊：
影响因子：
--
通讯作者：
Ohya Y
中科院分区：
文献类型：
--
作者：
Zhao T;Chida A;Shichino Y;Choi D;Mizunuma M;Iwasaki S;Ohya Y
在酵母的复制老化过程中，蛋白质合成受到严格的调控，但在复制老化过程中，全球翻译调控的特征却很少。为了在复制衰老过程中进行核糖体分析，我们使用微型化学调节老化装置收集了大量分裂的衰老细胞。翻译效率，定义为核糖体足迹的数量标准化转录丰度，比较每个基因的年轻和老年细胞。我们确定了700多个基因在复制衰老过程中发生了超过两倍的变化。在参与DNA修复和染色体组织的基因中观察到翻译效率的提高。编码核糖体成分的基因、转座子Ty1和Ty2基因、与未折叠蛋白反应相关的转录因子HAC1基因、参与细胞壁合成和组装的基因以及渗透氨酶基因的翻译效率均有所下降。我们的研究结果提供了复制衰老过程中翻译调控的全局视图，其中涉及各种细胞功能的途径受到翻译调控并导致多种表型变化。
Protein synthesis is strictly regulated during replicative aging in yeast, but global translational regulation during replicative aging is poorly characterized. To conduct ribosome profiling during replicative aging, we collected a large number of dividing aged cells using a miniature chemostat aging device. Translational efficiency, defined as the number of ribosome footprints normalized to transcript abundance, was compared between young and aged cells for each gene. We identified more than 700 genes with changes greater than twofold during replicative aging. Increased translational efficiency was observed in genes involved in DNA repair and chromosome organization. Decreased translational efficiency was observed in genes encoding ribosome components, transposon Ty1 and Ty2 genes, transcription factor HAC1 gene associated with the unfolded protein response, genes involved in cell wall synthesis and assembly, and ammonium permease genes. Our results provide a global view of translational regulation during replicative aging, in which the pathways involved in various cell functions are translationally regulated and cause diverse phenotypic changes.
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影响因子：
14.9
作者：
Gene Ontology Consortium
通讯作者：
Gene Ontology Consortium
DOI：
10.1007/978-1-0716-0592-9_1
发表时间：
2020
期刊：
Methods in molecular biology (Clifton, N.J.)
影响因子：
--
作者：
Yu R;Jo MC;Dang W
通讯作者：
Dang W
DOI：
10.1042/bcj20210432
发表时间：
2021-12-22
期刊：
The Biochemical journal
影响因子：
--
作者：
Campion R;Bloxam L;Burrow K;Brownridge PJ;Pentland DR;Thomas P;Gourlay CW;Eyers CE;Barclay JW;Morgan A
通讯作者：
Morgan A
影响因子：
64.5
作者：
Hughes, Casey E.;Coody, Troy K.;Hughes, Adam L.
通讯作者：
Hughes, Adam L.
蛋白质生物发生机制是酵母复制衰老的驱动力。
影响因子：
7.7
作者：
Janssens GE;Meinema AC;González J;Wolters JC;Schmidt A;Guryev V;Bischoff R;Wit EC;Veenhoff LM;Heinemann M
通讯作者：
Heinemann M