Contact-free determination of material characteristics using a newly developed pressure-operated strain-applying bioreactor.
Contact-free determination of material characteristics using a newly developed pressure-operated strain-applying bioreactor.
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使用新开发的压力操作应变应用生物反应器非接触式测定材料特性。
DOI：
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发表时间：
2008
期刊：
影响因子：
--
通讯作者：
J. Guttmann
中科院分区：
文献类型：
--
作者：
S. Schumann;C. Stahl;K. Möller;Matthias Schneider;R. Metzke;W. Wall;H. Priebe;J. Guttmann
生物样品的实验力学刺激有助于理解力学生物学的基本过程。不幸的是，目前的技术不允许对材料特性进行无接触的研究。我们开发了一种机械刺激器，其工作原理是假设放置在载体膜上的生物样品产生的反作用力可以从总信号中提取出来。由于生物样品的材料特性不明确，我们用聚氨酯和乳胶膜代替它们。基于之前所测载体膜顺应性的知识，我们根据生物反应器内的压力-体积关系计算了测试膜的顺应性。我们假设知道载体膜的顺应性可以计算样品的顺应性。乳胶膜的顺应性大于聚氨酯膜（p < 0.05）。在静态和动态机械刺激下，计算出的顺应性与直接测定的顺应性的平均差异分别为3.9% +/- 2.7%和6.7% +/- 4.8%（平均+/- SD） （p > 0.05）。与聚氨酯对乳胶组合相比，胶乳对聚氨酯的顺应性值的可变性大几倍（p < 0.01）。静力柔度估算和拉力试验机单轴拉伸测定的杨氏模量之间存在高度相关性（r(2) = 0.987, p < 0.001）。总之，我们的机械刺激器允许可靠的无接触测定材料特性，因此可能适用于生物样品的无接触评估。载体膜的顺应性应至少比被测材料的顺应性大两倍。
Experimental mechanostimulation of biologic samples serves to understand fundamental processes in mechanobiology. Unfortunately, present techniques do not allow contact-free investigation of material characteristics. We developed a mechanostimulator that functions under the assumption that the counterforce generated by a biologic sample placed on a carrier membrane (test-on-carrier) is extractable from the total signal. As material characteristics of biologic samples are poorly defined, we substituted them by polyurethane and latex membranes. Based on the knowledge of the previously measured carrier membrane's compliance, we calculated the test membrane's compliance from the pressure-volume relationship inside the bioreactor. We hypothesized that knowing the carrier membranes' compliance allows calculation of the sample's compliance. Compliances of latex membranes were larger than those of polyurethane ones (p < 0.05). The average differences between calculated and directly determined compliances were 3.9% +/- 2.7% and 6.7% +/- 4.8% (mean +/- SD) under static and dynamic mechanostimulation, respectively (p > 0.05). The variability of compliance values was several fold larger (p < 0.01) for latex-on-polyurethane compared to polyurethane-on-latex combinations. There was high correlation between Young's modulus as determined from static compliance estimation and by uniaxial stretching in a tension-testing machine (r(2) = 0.987, p < 0.001). In conclusion, our mechanostimulator allowed reliable contact-free determination of material characteristics and may thus be suitable for contact-free assessment of biologic samples. The compliance of the carrier membrane should be at least two times larger than the compliance of the tested material.
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影响因子：
4
作者：
A. Banes;J. Gilbert;D. Taylor;O. Monbureau
通讯作者：
A. Banes;J. Gilbert;D. Taylor;O. Monbureau
影响因子：
3.3
作者：
F. K. Winston;E. Macarak;S. Gorfien;Lawrence E. Thibault
通讯作者：
F. K. Winston;E. Macarak;S. Gorfien;Lawrence E. Thibault
影响因子：
3.3
作者：
Sanchez-Esteban, J;Cicchiello, LA;Rubin, LP
通讯作者：
Rubin, LP
影响因子：
7
作者：
Fermor, B;Jeffcoat, D;Guilak, F
通讯作者：
Guilak, F