Differences of reactive oxygen species metabolism in top, middle and bottom part of epicarp and mesocarp influence tomato fruit cracking
Differences of reactive oxygen species metabolism in top, middle and bottom part of epicarp and mesocarp influence tomato fruit cracking
复制标题
外果皮和中果皮上、中、下部分活性氧代谢差异对番茄裂果的影响
DOI：
10.1080/14620316.2020.1748525
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发表时间：
2020-04
影响因子：
1.9
通讯作者：
Yu Lu
中科院分区：
文献类型：
--
作者：
Zhang Chuan;Zhao Yingjie;Jiang Fangling;Wu Zhen;Cui Shouyao;Lv Haimeng;Yu Lu
摘要 番茄裂果是一个严重的经济问题，影响果实品质和产量。非生物胁迫期间，活性氧（ROS）代谢失衡导致膜损伤，可能会增加果皮对环境变化的敏感性，进而影响果实裂果。为了进一步验证ROS代谢与裂果之间的关系，我们以抗裂番茄‘LA1698’和易裂番茄‘LA2683’为试验材料。研究了外果皮和中果皮不同部位（顶部、中部和底部）的ROS、膜脂过氧化、抗氧化剂和可溶性蛋白。结果表明，‘LA2683’外果皮顶部ROS含量和膜脂过氧化程度最高。此外，‘LA2683’外果皮各部位的超氧化物歧化酶（SOD）、抗坏血酸过氧化物酶（APX）、谷胱甘肽还原酶（GR）活性和抗坏血酸（AsA）含量均较低，而外果皮各部位的多酚氧化酶（PPO）、过氧化物酶（POD）、过氧化氢酶（CAT）活性和可溶性蛋白含量均高于 ‘LA1698’。综上所述，‘LA2683’果皮顶部ROS积累量较高，同时ROS清除能力下降，ROS稳态紊乱导致细胞氧化损伤，进而影响果实顶部裂裂的发生。外果皮中的ROS代谢可能在果实裂果中发挥重要作用。
ABSTRACT Tomato (Solanum lycopersicum L.) fruit cracking is a serious economic problem, affecting fruit quality and yield. During abiotic stress, the imbalance of reactive oxygen species (ROS) metabolism causing membrane damage might increase the susceptibility of pericarp to changing environment and then affect fruit cracking. To further validate the relationship between ROS metabolism and fruit cracking, we used cracking-resistant tomato ‘LA1698’ and cracking-susceptible tomato ‘LA2683’ as test materials. ROS, membrane lipid peroxidation, antioxidants and soluble protein in different parts (top, middle and bottom) of epicarp and mesocarp were studied. Results showed that, the highest ROS levels and membrane lipid peroxidation degree were observed in the top of epicarp in ‘LA2683’. Moreover, ‘LA2683’ displayed lower superoxide dismutase (SOD), ascorbate peroxidase (APX), glutathione reductase (GR) activity and ascorbic acid (AsA) content, and maintained higher polyphenol oxidase (PPO), peroxidase (POD), catalase (CAT) activity and soluble protein content in each part of epicarp, compared with ‘LA1698’. In conclusion, the top of epicarp in ‘LA2683’ accumulated higher ROS, concurrent with decreased ROS scavenging ability, and the disordered ROS homoeostasis led to cellular oxidative damage, which could effect the occurrence of cracking at the top of fruit. ROS metabolism in epicarp may play an important role in fruit cracking.
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影响因子：
6.9
作者：
Czerednik A;Busscher M;Bielen BA;Wolters-Arts M;de Maagd RA;Angenent GC
通讯作者：
Angenent GC
DOI：
10.1007/s11703-007-0010-2
发表时间：
2007-02
期刊：
Frontiers of Agriculture in China
影响因子：
--
作者：
Xue-zheng Wang;Hua Wang;Fengzhi Wu;Bo Liu
通讯作者：
Xue-zheng Wang;Hua Wang;Fengzhi Wu;Bo Liu
影响因子：
4.9
作者：
Y. Nakano;K. Asada
通讯作者：
Y. Nakano;K. Asada
影响因子：
2.9
作者：
Shan, Changjuan;Zhang, Shengli;Ou, Xingqi
通讯作者：
Ou, Xingqi
影响因子：
4.3
作者：
L. Sekse
通讯作者：
L. Sekse