Regulation of tomato fruit pericarp development by an interplay between CDKB and CDKA1 cell cycle genes.
Regulation of tomato fruit pericarp development by an interplay between CDKB and CDKA1 cell cycle genes.
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DOI：
10.1093/jxb/err451
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发表时间：
2012-04
影响因子：
6.9
通讯作者：
Angenent GC
中科院分区：
文献类型：
--
作者：
Czerednik A;Busscher M;Bielen BA;Wolters-Arts M;de Maagd RA;Angenent GC
番茄果实的生长是由细胞分裂和细胞扩张决定的，这些细胞分裂和细胞扩张受到驱动核心细胞周期的因素的严格控制。细胞周期蛋白依赖性激酶（CDK）及其相互作用伙伴细胞周期蛋白在细胞周期的进程中起着关键作用。在这项研究中，CDKA 1，CDKB 1和CDKB 2在果实发育中的作用的特点是果实特异性过表达和下调。CDKA 1在整个发育过程中在果皮中表达，但在生长期结束时，当CDKB基因表达停止时，在外部果皮细胞层中强烈上调。在发育后期CDKB基因的过表达和CDKA 1的下调导致非常相似的果实表型，表现为果皮中细胞层数的减少和果实干燥的改变。表达研究表明，CDKB 1和CDKB 2过表达突变体中CDKB 1/2的表达下调CDKA 1，这表明这些类型的CDK蛋白在番茄果皮发育中的相反作用。
Growth of tomato fruits is determined by cell division and cell expansion, which are tightly controlled by factors that drive the core cell cycle. The cyclin-dependent kinases (CDKs) and their interacting partners, the cyclins, play a key role in the progression of the cell cycle. In this study the role of CDKA1, CDKB1, and CDKB2 in fruit development was characterized by fruit-specific overexpression and down-regulation. CDKA1 is expressed in the pericarp throughout development, but is strongly up-regulated in the outer pericarp cell layers at the end of the growth period, when CDKB gene expression has ceased. Overexpression of the CDKB genes at later stages of development and the down-regulation of CDKA1 result in a very similar fruit phenotype, showing a reduction in the number of cell layers in the pericarp and alterations in the desiccation of the fruits. Expression studies revealed that CDKA1 is down-regulated by the expression of CDKB1/2 in CDKB1 and CDKB2 overexpression mutants, suggesting opposite roles for these types of CDK proteins in tomato pericarp development.
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影响因子：
7.4
作者：
Cheniclet, C;Rong, WY;Renaudin, JP
通讯作者：
Renaudin, JP
影响因子：
4.3
作者：
DELAAT, AMM;VERHOEVEN, HA;DIJKHUIS, P
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DIJKHUIS, P
影响因子：
5.2
作者：
Bergervoet, JHW;Verhoeven, HA;Bino, RJ
通讯作者：
Bino, RJ
影响因子：
11.6
作者：
GILLASPY, G;BENDAVID, H;GRUISSEM, W
通讯作者：
GRUISSEM, W
影响因子：
11.6
作者：
Imai, KK;Ohashi, Y;Aoyama, T
通讯作者：
Aoyama, T