Stability of person-specific blood-based infrared molecular fingerprints opens up prospects for health monitoring.
Stability of person-specific blood-based infrared molecular fingerprints opens up prospects for health monitoring.
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DOI：
10.1038/s41467-021-21668-5
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发表时间：
2021-03-08
影响因子：
16.6
通讯作者：
Žigman M
中科院分区：
文献类型：
--
作者：
Huber M;Kepesidis KV;Voronina L;Božić M;Trubetskov M;Harbeck N;Krausz F;Žigman M
健康状态转变反映在生物流体的分子组成的特征变化中。在广泛的分子种类中并行检测这些变化，可能有助于检测异常生理学。通过红外振动光谱对生物流体进行指纹识别提供了这种能力。红外线在健康监测方面的潜力是否真的能被利用，关键取决于个人的红外线分子指纹（IMF）随着时间的推移有多稳定。在这里，我们报告了一项解决这个问题的概念验证研究。使用傅里叶变换红外光谱，我们指纹的血清和血浆样品从31个健康的，无症状的个人，谁采样多达13次，在7周内，并再次在6个月后。测量直接在液体血清和血浆样品上进行，产生了时间和成本效益的工作流程和高度的再现性。发现所得IMF在临床相关时间尺度上高度稳定。单次测量产生了多个人的特定光谱标记，允许个人的分子表型进行检测，并随着时间的推移。这种以前未知的个人生化指纹的时间稳定性形成了基于血液的红外光谱指纹作为基于多组学的健康监测模式的未来应用的基础。健康状态转变反映为生物流体的分子组成的特征变化。在这里，作者应用红外分子指纹技术，揭示了血液表型随着时间的推移是足够稳定的，为时间和成本效益的健康监测提供了基础。
Health state transitions are reflected in characteristic changes in the molecular composition of biofluids. Detecting these changes in parallel, across a broad spectrum of molecular species, could contribute to the detection of abnormal physiologies. Fingerprinting of biofluids by infrared vibrational spectroscopy offers that capacity. Whether its potential for health monitoring can indeed be exploited critically depends on how stable infrared molecular fingerprints (IMFs) of individuals prove to be over time. Here we report a proof-of-concept study that addresses this question. Using Fourier-transform infrared spectroscopy, we have fingerprinted blood serum and plasma samples from 31 healthy, non-symptomatic individuals, who were sampled up to 13 times over a period of 7 weeks and again after 6 months. The measurements were performed directly on liquid serum and plasma samples, yielding a time- and cost-effective workflow and a high degree of reproducibility. The resulting IMFs were found to be highly stable over clinically relevant time scales. Single measurements yielded a multiplicity of person-specific spectral markers, allowing individual molecular phenotypes to be detected and followed over time. This previously unknown temporal stability of individual biochemical fingerprints forms the basis for future applications of blood-based infrared spectral fingerprinting as a multiomics-based mode of health monitoring. Health status transitions are reflected as characteristic changes in molecular composition of biofluids. Here, the authors apply infrared molecular fingerprinting and reveal that blood-based phenotypes are sufficiently stable over time, providing the basis for time- and cost-effective health monitoring.
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影响因子：
5.2
作者：
Draisma, Harmen H. M.;Reijmers, Theo H.;Boomsma, Dorret I.
通讯作者：
Boomsma, Dorret I.
影响因子：
3.5
作者：
Fabian, H;Lasch, P;Naumann, D
通讯作者：
Naumann, D
影响因子：
7.3
作者：
Gazi, E;Dwyer, J;Brown, M
通讯作者：
Brown, M
DOI：
10.1073/pnas.0705685105
发表时间：
2008-02-05
影响因子：
11.1
作者：
Assfalg, Michael;Bertini, Ivano;Spraul, Manfred
通讯作者：
Spraul, Manfred
影响因子：
4
作者：
Carmona, Pedro;Molina, Marina;Toledanog, Adolfo
通讯作者：
Toledanog, Adolfo