Mild/asymptomatic COVID-19 in unvaccinated pregnant mothers impairs neonatal immune responses.
Mild/asymptomatic COVID-19 in unvaccinated pregnant mothers impairs neonatal immune responses.
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DOI：
10.1172/jci.insight.172658
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发表时间：
2023-09-12
期刊：
影响因子：
8
通讯作者：
Messaoudi, Ilhem
中科院分区：
文献类型：
--
作者：
Doratt, Brianna M.;Sureshchandra, Suhas;True, Heather;Rincon, Monica;Marshall, Nicole E.;Messaoudi, Ilhem
母体感染SARS-CoV-2会引发胎盘炎症并改变脐带血免疫细胞组成。然而，大多数研究都集中在严重母体感染的结果上。因此，我们分析了未接种疫苗的母亲在怀孕期间经历轻度/无症状SARS-CoV-2感染的新生儿的脐带血和绒毛膜绒毛。我们使用流式细胞术、单细胞RNA测序和TLR激动剂和病原体体外刺激的功能读数来研究免疫细胞重新布线。母体感染与脐带血中记忆性T细胞、B细胞和非经典单核细胞的频率增加有关。体外T细胞和B细胞对刺激的反应减弱，表明存在耐受性状态。在脐带血单核细胞中也观察到适应不良反应，其中抗病毒反应被抑制，但对细菌tlr的反应增加。母体感染还与胎盘霍夫鲍尔细胞的扩增和激活有关，分泌高水平的髓细胞募集趋化因子。此外，我们还报道了母体来源的单核细胞/巨噬细胞在胎儿胎盘中的激活增加，这些细胞被转录为抗病毒应答。我们的数据表明，即使在新生儿没有垂直传播或症状的情况下，轻度/无症状的母体COVID-19也会改变胎儿循环和胎盘中免疫细胞的转录和功能状态。
Maternal SARS-CoV-2 infection triggers placental inflammation and alters cord blood immune cell composition. However, most studies focus on outcomes of severe maternal infection. Therefore, we analyzed cord blood and chorionic villi from newborns of unvaccinated mothers who experienced mild/asymptomatic SARS-CoV-2 infection during pregnancy. We investigated immune cell rewiring using flow cytometry, single-cell RNA sequencing, and functional readouts using ex vivo stimulation with TLR agonists and pathogens. Maternal infection was associated with increased frequency of memory T and B cells and nonclassical monocytes in cord blood. Ex vivo T and B cell responses to stimulation were attenuated, suggesting a tolerogenic state. Maladaptive responses were also observed in cord blood monocytes, where antiviral responses were dampened but responses to bacterial TLRs were increased. Maternal infection was also associated with expansion and activation of placental Hofbauer cells, secreting elevated levels of myeloid cell–recruiting chemokines. Moreover, we reported increased activation of maternally derived monocytes/macrophages in the fetal placenta that were transcriptionally primed for antiviral responses. Our data indicate that even in the absence of vertical transmission or symptoms in the neonate, mild/asymptomatic maternal COVID-19 altered the transcriptional and functional state in fetal immune cells in circulation and in the placenta.
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影响因子：
7.2
作者：
Beck C;Gallagher K;Taylor LA;Goldstein JA;Mithal LB;Gernand AD
通讯作者：
Gernand AD
DOI：
10.3390/v13061062
发表时间：
2021-06-03
期刊：
Viruses
影响因子：
--
作者：
Callahan V;Hawks S;Crawford MA;Lehman CW;Morrison HA;Ivester HM;Akhrymuk I;Boghdeh N;Flor R;Finkielstein CV;Allen IC;Weger-Lucarelli J;Duggal N;Hughes MA;Kehn-Hall K
通讯作者：
Kehn-Hall K
影响因子：
2.6
作者：
通讯作者：
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胎儿运动减少：胎盘脑损伤感染胎盘SARS-COV-2的迹象。
DOI：
10.3390/v13122517
发表时间：
2021-12-15
期刊：
Viruses
影响因子：
--
作者：
Favre G;Mazzetti S;Gengler C;Bertelli C;Schneider J;Laubscher B;Capoccia R;Pakniyat F;Ben Jazia I;Eggel-Hort B;de Leval L;Pomar L;Greub G;Baud D;Giannoni E
通讯作者：
Giannoni E
影响因子：
3.6
作者：
Duan, Liyan;Reisch, Beatrix;Iannaccone, Antonella
通讯作者：
Iannaccone, Antonella