Characterization of the main magnesium transporters mediating different Mg translocation from root to shoot between Mg-tolerant and Mg-sensitive Brassica napus cultivars under magnesium deficiency stress
Characterization of the main magnesium transporters mediating different Mg translocation from root to shoot between Mg-tolerant and Mg-sensitive Brassica napus cultivars under magnesium deficiency stress
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缺镁胁迫下介导耐镁和镁敏感甘蓝型油菜品种之间不同镁从根到芽转运的主要镁转运蛋白的表征
DOI：
10.1007/s11104-019-04303-7
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发表时间：
2019-10
期刊：
影响因子：
4.9
通讯作者：
Nannan Li
中科院分区：
文献类型：
--
作者：
Juanjuan Sun;Shengting Li;Shufeng Wang;Zhongchun Xiao;Zhen Zhang;Jiana Li;Feng Luo;Nannan Li
镁（Mg）缺乏影响甘蓝型油菜（B. napus）的许多代谢过程，导致产量损失。然而，甘蓝型油菜对Mg2+的吸收和转运机制尚不清楚。在缺镁条件下筛选了39个基因型的甘蓝型油菜，根据其根部到茎部的Mg转移因子（TF）选择了品种P160和P153。我们通过分析叶片叶绿素浓度、丙二醛和过氧化物酶活性以及还原糖浓度进一步对这两个基因型进行了特征分析。此外，我们对P153和P160的茎和根进行了转录组学和qRT-PCR分析。通过酵母和拟南芥突变体的功能分析，鉴定了参与镁转运的功能基因。生理分析表明，耐镁品种P160 （Mg - t）在缺镁环境下的耐受性优于敏感品种P153 （Mg - s）。转录组学和qRT-PCR分析结果显示，Mg- t品种茎部BnMGT1-2和BnMGT6-1转录本表达量在Mg限制下比Mg- s品种表达量显著上调。对BnMGT1-2和BnMGT6-1的功能分析表明，BnMGT1-2和BnMGT6-1是在低镁条件下介导镁从根向茎部转运的两个主要功能镁转运体。Mg- t比Mg- s更有效地将Mg从根转运到地上部。在缺镁条件下，BnMGT1-2和BnMGT6-1可能是与Mg转运相关的两个主要转运蛋白，导致Mg- t和Mg- s的Mg效率存在差异。
Magnesium (Mg) deficiency impacts many metabolic processes in Brassica napus (B. napus), leading to yield loss. However, the mechanism of Mg2+ uptake and translocation in B. napus remains unknown. After screening 39 genotypes of B. napus under Mg-deficient conditions, the cultivars P160 and P153 were selected according to their Mg transfer factor (TF) from root to shoot. We further characterized these two genotypes under Mg-deficiency by analyzing chlorophyll concentration, malondialdehyde and peroxidase activity, and reducing sugar concentration in leaves. Additionally, we performed transcriptomics and qRT-PCR assays on P153 and P160 shoots and roots. The identified functional genes involved in Mg transport were characterized by functional assays in yeast and Arabidopsis mutants. The physiological analysis revealed that P160 (Mg tolerance cultivar; Mg-T) is more tolerant than P153 (Mg sensitive cultivar; Mg-S) under magnesium-deficient environments. Transcriptomics and qRT-PCR assays revealed that transcript levels of BnMGT1–2 and BnMGT6–1 were more significantly up-regulated in the shoot of Mg-T cultivar than that of the Mg-S cultivar under Mg limitation. Functional assays of BnMGT1–2 and BnMGT6–1 reveals that BnMGT1–2 and BnMGT6–1 are the two main functional Mg transporters mediating Mg translocation from root to shoot under low Mg conditions. Mg-T is more efficient in the translocation of Mg from root to shoot than Mg-S. BnMGT1–2 and BnMGT6–1 should be the two main Mg transporters associated with Mg translocation under Mg deficiency condition, which caused the different Mg efficiency between the Mg-T and Mg-S.
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影响因子：
1.8
作者：
Sangha JS;Gu K;Kaur J;Yin Z
通讯作者：
Yin Z
影响因子：
3.2
作者：
HMIEL, SP;SNAVELY, MD;MAGUIRE, ME
通讯作者：
MAGUIRE, ME
DOI：
10.1002/9781118453926.ch22
发表时间：
2013-07
期刊：
--
影响因子：
--
作者：
M. Favus;D. Goltzman
通讯作者：
M. Favus;D. Goltzman
影响因子：
4.4
作者：
Jiang Y;Li Y;Lee W;Xu X;Zhang Y;Zhao R;Zhang Y;Wang W
通讯作者：
Wang W
拟南芥转运蛋白 MGT6 介导镁吸收，是镁限制下生长所必需的
影响因子：
11.6
作者：
Mao, Dandan;Chen, Jian;Luan, Sheng
通讯作者：
Luan, Sheng