MicroRNA sequence analysis identifies microRNAs associated with peri-implantitis in dogs
MicroRNA sequence analysis identifies microRNAs associated with peri-implantitis in dogs
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MicroRNA 序列分析识别与狗种植体周围炎相关的 microRNA
DOI：
10.1042/bsr20170768
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发表时间：
2017-09
影响因子：
4
通讯作者：
Huang Hui
中科院分区：
文献类型：
--
作者：
Wu Xiaolin;Chen Xipeng;Mi Wenxiang;Wu Tingting;Gu Qinhua;Huang Hui
种植体周围炎以致密的炎性细胞浸润和破骨细胞活性增加为特征，可导致牙槽骨破坏和种植失败。MiRNAs参与各种炎症性疾病的调节，如牙周炎和骨质疏松症。因此，本研究旨在研究miRNAs在犬种植体周围炎中的差异表达，并探讨其靶基因的功能。通过miRNA序列分析鉴定种植周炎组织中差异表达的miRNAs。根据折叠变化的标准>；1.5和P<；0.01，选择上调的8个和下调的30个miRNAs用于预测靶基因及其生物学功能。基于基因本体论(GO)和KEGG通路分析的结果，这些miRNAs可能通过复杂的机制微调种植体周围炎的炎症过程。实时定量聚合酶链式反应(qRT-PCR)结果显示，let-7G、miR-27A和miR-145可能在种植体周围炎中起重要作用，值得进一步研究。本研究的结果为深入了解差异表达的miRNAs的潜在生物学效应提供了深入的见解，并观察到参与丝裂原活化蛋白激酶(MAPK)信号通路的靶基因的特异性丰富。这些发现突出了miRNAs在炎症和破骨细胞形成中的复杂和特定的作用，这两个方面都是种植体周围炎的关键方面，因此可能有助于未来对种植体周围炎的病因、潜在机制和治疗的研究。
Peri-implantitis, which is characterized by dense inflammatory infiltrates and increased osteoclast activity, can lead to alveolar bone destruction and implantation failure. miRNAs participate in the regulation of various inflammatory diseases, such as periodontitis and osteoporosis. Therefore, the present study aimed to investigate the differential expression of miRNAs in canine peri-implantitis and to explore the functions of their target genes. An miRNA sequence analysis was used to identify differentially expressed miRNAs in peri-implantitis. Under the criteria of a fold-change >1.5 and P<0.01, 8 up-regulated and 30 down-regulated miRNAs were selected for predictions of target genes and their biological functions. Based on the results of Gene Ontology (GO) and KEGG pathway analyses, these miRNAs may fine-tune the inflammatory process in peri-implantitis through an intricate mechanism. The results of quantitative real-time PCR (qRT-PCR) revealed that let-7g, miR-27a, and miR-145 may play important roles in peri-implantitis and are worth further investigation. The results of the present study provide insights into the potential biological effects of the differentially expressed miRNAs, and specific enrichment of target genes involved in the mitogen-activated protein kinase (MAPK) signaling pathway was observed. These findings highlight the intricate and specific roles of miRNAs in inflammation and osteoclastogenesis, both of which are key aspects of peri-implantitis, and thus may contribute to future investigations of the etiology, underlying mechanism, and treatment of peri-implantitis.
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Let-7e通过ceRNA串扰调节血管内皮细胞的炎症反应
影响因子：
4.6
作者：
Lin Z;Ge J;Wang Z;Ren J;Wang X;Xiong H;Gao J;Zhang Y;Zhang Q
通讯作者：
Zhang Q
影响因子：
4.4
作者：
Martinez, Fernando O.;Gordon, Siamon;Mantovani, Alberto
通讯作者：
Mantovani, Alberto
影响因子：
6.7
作者：
Berglundh, Tord;Zitzmann, Nicola U.;Donati, Mauro
通讯作者：
Donati, Mauro
DOI：
10.1615/jlongtermeffmedimplants.2013007073
发表时间：
2013
影响因子：
--
作者：
M. Kadkhodazadeh;A. Jafari;R. Amid;A. Ebadian;M. Alipour;Fatemeh Mollaverdi;A. Lafzi
通讯作者：
M. Kadkhodazadeh;A. Jafari;R. Amid;A. Ebadian;M. Alipour;Fatemeh Mollaverdi;A. Lafzi
影响因子：
64.8
作者：
Suzuki, N;Suzuki, S;Yeh, WC
通讯作者：
Yeh, WC