Antimicrobial Efficacy of Triple Antibiotic-eluting Polymer Nanofibers against Multispecies Biofilm.
Antimicrobial Efficacy of Triple Antibiotic-eluting Polymer Nanofibers against Multispecies Biofilm.
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DOI：
10.1016/j.joen.2017.06.009
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发表时间：
2017-09
影响因子：
4.2
通讯作者：
Bottino MC
中科院分区：
文献类型：
--
作者：
Albuquerque MTP;Nagata J;Bottino MC
消除未成熟恒牙坏死牙髓的微生物菌群是再生治疗长期成功的关键，也是一个具有挑战性的目标。最近的研究集中在开发细胞友好的管内给药系统。本实验旨在探讨三维管状三层抗菌洗脱纳米纤维结构物对牙本质多物种生物膜的抗菌作用。聚二氧环酮（PDS）聚合物溶液，不含抗生素或与甲硝唑、环丙沙星和米诺环素结合，电纺成三维管状结构。在体外牙本质切片模型中，通过离心将由naeslundii放线菌、血链球菌和粪肠球菌组成的多物种生物膜强制放入牙本质小管中。将感染标本分为两个实验组；即三维管状三抗生素洗脱构建体和TAP，以及两个对照组（未经7天生物膜处理和不含抗生素的三维管状构建体）。用共聚焦激光扫描显微镜（CLSM）定量分析生物膜消除情况。CLSM分析显示，有大量的活菌（绿色）粘附在牙本质上并渗透到牙本质小管中。三维管状纳米纤维结构暴露后，CLSM图像显示，牙本质表面和牙本质小管内的活菌几乎完全消失，这与TAP组促进的细菌死亡相似（p<0.05），但与不含抗生素的三维管状纳米纤维结构和对照组（生理盐水）相比，明显更高。所提出的3D管状抗生素洗脱结构对所测试的多物种生物膜显示出明显的抗菌效果，因此作为再生牙髓学之前的消毒策略具有重要的临床潜力。
Elimination of microbial flora in cases of immature permanent teeth with necrotic pulp is both key and a challenging goal for the long-term success of regenerative therapy. Recent research has focused on the development of cell-friendly intracanal drug delivery systems. This in vitro study aimed to investigate the antimicrobial action of three-dimensional (3D) tubular-shaped triple antibiotic-eluting nanofibrous constructs against a multispecies biofilm on human dentin. Polydioxanone (PDS) polymer solutions, antibiotic-free or incorporated with metronidazole, ciprofloxacin, and minocycline were electrospun into 3D tubular-shaped constructs. A multispecies biofilm consisting of Actinomyces naeslundii, Streptococcus sanguinis, and Enterococcus faecalis, was forced inside the dentinal tubules via centrifugation in a dentin slice in vitro model. The infected specimens were exposed to two experimental groups; i.e., 3D tubular-shaped triple antibiotic-eluting constructs and TAP, and two control groups (7-day biofilm untreated and antibiotic-free 3D tubular-shaped constructs). Biofilm elimination was quantitatively analyzed by Confocal Laser Scanning Microscopy (CLSM). CLSM analysis showed a dense population of viable (green) bacteria adhered to dentin and penetrated into the dentinal tubules. Upon 3D tubular-shaped triple antibiotic-eluting nanofibrous construct exposure, nearly complete elimination of viable bacteria on the dentin surface and inside the dentinal tubules was shown by the CLSM images, which was similar (p<0.05) to the bacterial death promoted by the TAP group but significantly greater when compared to both the antibiotic-free 3D tubular-shaped constructs and the control (saline). The proposed 3D tubular-shaped antibiotic-eluting construct showed pronounced antimicrobial effects against the multispecies biofilm tested and therefore holds significant clinical potential as a disinfection strategy prior to regenerative endodontics.
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影响因子：
4.2
作者：
Hargreaves KM;Diogenes A;Teixeira FB
通讯作者：
Teixeira FB
影响因子：
4.2
作者：
Du, Tianfeng;Wang, Zhejun;Haapasalo, Markus
通讯作者：
Haapasalo, Markus
抗菌TAP-MIMIC电纺聚合物支架：对牙龈疟原虫感染的牙本质生物膜的影响。
影响因子：
3.4
作者：
Albuquerque MT;Evans JD;Gregory RL;Valera MC;Bottino MC
通讯作者：
Bottino MC
影响因子：
3.4
作者：
Bottino, Marco C.;Arthur, Rodrigo A.;Waeiss, R. Aaron;Kamocki, Krzysztof;Gregson, Karen S.;Gregory, Richard L.
通讯作者：
Gregory, Richard L.
影响因子：
15.9
作者：
Jenkinson, Howard F.
通讯作者：
Jenkinson, Howard F.