Distinct mechanisms of Up state maintenance in the medial entorhinal cortex and neocortex.
Distinct mechanisms of Up state maintenance in the medial entorhinal cortex and neocortex.
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内侧内部皮层和新皮层中UP状态维持的不同机制。
DOI：
10.1016/j.neuropharm.2016.11.009
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发表时间：
2017-02
影响因子：
4.7
通讯作者：
Magloire V
中科院分区：
文献类型：
--
作者：
Digby RJ;Bravo DS;Paulsen O;Magloire V
内侧内嗅皮层是控制海马和新皮层之间信息交流的关键结构。在慢波睡眠期间，它表现出比新皮层Up状态更持久的持续活动，从而使内嗅皮层-海马体相互作用与新皮层脱钩，从而从其他皮层区域中脱颖而出。在这里，我们比较了机制，在桶皮质（BC）和MEC使用全细胞记录急性小鼠脑切片中的向上状态的维护。浴应用的NMDA受体拮抗剂取消了Up状态在BC，并减少了发病率，但不持续时间的Up状态在mEC。相反，红藻氨酸受体的封锁减少了最高状态的持续时间在mEC，但不是在BC。电压钳记录表明，非NMDA谷氨酸受体介导的慢兴奋性突触后电流的存在下，敏感的选择性红藻氨酸受体拮抗剂UBP-302，在第三层的mEC，这是没有观察到在BC的神经元。此外，我们发现，红藻氨酸受体介导的电流有助于恢复到最高状态的膜电位后，电流诱导的超极化的个别细胞中的mEC。最后，我们能够在仅由AMPA和红藻氨酸受体介导的电流支持的指数积分和放电神经元的网络模型中产生Up状态活动。我们建议，突触红藻氨酸受体负责的独特性能的mEC的状态。在体外，在没有新皮层输入的情况下，mEC表现出持续的Up状态活动。NMDA受体是新皮层中Up状态所必需的，但不是在mEC中。红藻氨酸受体在mEC中维持Up状态。红藻氨酸受体电流足以在计算机上维持Up状态。
The medial entorhinal cortex (mEC) is a key structure which controls the communication between the hippocampus and the neocortex. During slow-wave sleep, it stands out from other cortical regions by exhibiting persistent activity that outlasts neocortical Up states, decoupling the entorhinal cortex-hippocampal interaction from the neocortex. Here, we compared the mechanisms involved in the maintenance of the Up state in the barrel cortex (BC) and mEC using whole cell recordings in acute mouse brain slices. Bath application of an NMDA receptor antagonist abolished Up states in the BC, and reduced the incidence but not the duration of Up states in the mEC. Conversely, blockade of kainate receptors decreased Up state duration in the mEC, but not in the BC. Voltage clamp recordings demonstrated the presence of a non-NMDA glutamate receptor-mediated slow excitatory postsynaptic current, sensitive to the selective kainate receptor antagonist UBP-302, in layer III neurons of the mEC, which was not observed in the BC. Moreover, we found that kainate receptor-mediated currents assist in recovery back to the Up state membrane potential following a current-induced hyperpolarisation of individual cells in the mEC. Finally, we were able to generate Up state activity in a network model of exponential integrate-and-fire neurons only supported by AMPA and kainate receptor-mediated currents. We propose that synaptic kainate receptors are responsible for the unique properties of mEC Up states. mEC exhibits persistent Up state activity in the absence of neocortical input in vitro. NMDA receptors are required for Up states in the neocortex, but not in the mEC. Kainate receptors maintain Up states in the mEC. Kainate receptor currents are sufficient to maintain Up states in silico.
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影响因子：
15.9
作者：
Banerjee A;Larsen RS;Philpot BD;Paulsen O
通讯作者：
Paulsen O
影响因子：
2.5
作者：
Compte, A;Sanchez-Vives, MV;Wang, XJ
通讯作者：
Wang, XJ
影响因子：
3.5
作者：
Chamberlain, Sophie E. L.;Jane, David E.;Jones, Roland S. G.
通讯作者：
Jones, Roland S. G.
DOI：
10.1016/0736-5748(90)90011-p
发表时间：
1990-01-01
影响因子：
1.8
作者：
ERDO, SL;WOLFF, JR
通讯作者：
WOLFF, JR
DOI：
10.1007/bf02284847
发表时间：
1996-12-01
期刊：
JOURNAL OF NEUROCYTOLOGY
影响因子：
--
作者：
Feldmeyer, D;CullCandy, S
通讯作者：
CullCandy, S