Transcriptional response in rainbow trout (Oncorhynchus mykiss) B cells and thrombocytes following in vivo exposure to benzo[a]pyrene.
Transcriptional response in rainbow trout (Oncorhynchus mykiss) B cells and thrombocytes following in vivo exposure to benzo[a]pyrene.
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虹鳟鱼 (Oncorhynchus mykiss) B 细胞和血小板在体内接触苯并[a]芘后的转录反应
DOI：
10.1016/j.etap.2017.06.002
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发表时间：
2017
影响因子：
4.3
通讯作者：
van den Heuvel M.R.
中科院分区：
文献类型：
--
作者：
Phalen L.J;Köllner B;Hogan N.S;van den Heuvel M.R.
多环芳烃（PAHs）对鱼类的免疫毒性已被频繁报道，但其不同细胞毒性的原因尚不清楚。以虹鳟鱼为实验动物，分别腹腔注射玉米油和100 mg/kg的多环芳烃苯并[a]芘（B[a]P）。14天后从头肾、脾和血液中收获白细胞，这是先前研究中发现的B细胞去除的最佳时间。检测了B细胞和血小板中5种细胞色素P450（CYP）酶、芳烃受体（AhR）和内源性途径凋亡检查点（p53）的mRNA表达。在免疫磁性分离的B细胞和来自这些组织的血小板以及肝脏中测量转录水平，因为先前已经显示B细胞对BaP有响应，而血小板没有。在肝脏、血液B细胞、血液和脾脏血小板中诱导CYP 1A 1;在血液B细胞、血液和脾脏血小板中诱导CYP 1B 1;在肝脏、血液和脾脏B细胞、血液血小板中诱导CYP 1A 3;在肝脏中诱导CYP 1C 1;在肝脏和脾脏血小板中诱导AhR。不同组织或细胞类型的CYP 1C 2或p53 mRNA水平无变化。暴露后14天观察到mRNA的诱导，表明单次B[a]P注射的长期生理效应。CYP 1A 1和CYP 1A 3是最丰富表达的CYP 1B 1基因，而CYP 1B 1通常最不丰富。B[a] P诱导的血小板具有与肝细胞或B细胞显著不同的CD 4+表达模式。考虑到代谢物在多环芳烃毒性中的重要性，组织之间的TMPs表达的差异可以解释先前在B细胞和血小板之间观察到的毒性差异。
Immune toxicity of polycyclic aromatic hydrocarbons (PAHs) in fishes has been frequently reported but the reasons for differential cell toxicity remains unclear. Rainbow trout were exposed in vivo with a single intraperitoneal injection of corn oil or 100 mg/kg of the immunotoxic PAH benzo[a]pyrene (B[a]P) in corn oil. Leukocytes were harvested from head kidney, spleen and blood after 14 days, the optimal time for B cell depletion found in a previous study. The mRNA expression of five cytochrome P450 (CYP) enzymes, the aryl hydrocarbon receptor (AhR), and an intrinsic pathway apoptosis checkpoint (p53) in B cells and thrombocytes were examined. Transcript levels were measured in immunomagnetically-isolated B cells and thrombocytes from those tissues as well as in liver as B cells had been previously shown to be responsive the BaP whereas thrombocytes were not. There was induction of CYP1A1 in liver, blood B cells, and blood and spleen thrombocytes; CYP1B1 in blood B cells, blood and spleen thrombocytes; CYP1A3 in liver, blood and spleen B cells, and blood thrombocytes; CYP1C1 in liver; and AhR in liver and spleen thrombocytes. There was no change in CYP1C2, or p53 mRNA levels across tissues or cell type. Induction in mRNA was observed 14 d after exposure, indicating a prolonged physiological effect of a single B[a]P injection. CYP1A1 and CYP1A3 were the most abundantly expressed CYP genes and CYP1B1 was generally least abundant. B[a]P-induced thrombocytes had a significantly different pattern of CYP expression than either liver or B cells. Given the importance of metabolites in the toxicity of PAHs, differences in CYP expression between tissues may explain differences in toxicity previously observed between B cells and thrombocytes.
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