Extending the viability of acute brain slices.
Extending the viability of acute brain slices.
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DOI：
10.1038/srep05309
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发表时间：
2014-06-16
影响因子：
4.6
通讯作者：
Morley JW
中科院分区：
文献类型：
--
作者：
Buskila Y;Breen PP;Tapson J;van Schaik A;Barton M;Morley JW
急性脑片的寿命约为6-12 小时，限制了潜在的实验时间。我们设计了一种新的复苏孵化系统，能够将它们的寿命延长到36 小时以上。该系统控制孵化的人工脑脊液(ACSF)的温度，同时持续将液体通过UVC过滤系统，并同时监测温度和pH。控制温度和UVC过滤的组合将细菌水平保持在滞后阶段，并导致脑片寿命的大幅延长。脑片活性通过电生理记录和活/死细胞分析进行验证。该系统通过监测孵化条件和规范这种人工环境，使研究人员受益。它进一步提供了两个实验天的活性组织，减少了准备脑片所需的时间和研究所需的动物数量。
The lifespan of an acute brain slice is approximately 6–12 hours, limiting potential experimentation time. We have designed a new recovery incubation system capable of extending their lifespan to more than 36 hours. This system controls the temperature of the incubated artificial cerebral spinal fluid (aCSF) while continuously passing the fluid through a UVC filtration system and simultaneously monitoring temperature and pH. The combination of controlled temperature and UVC filtering maintains bacteria levels in the lag phase and leads to the dramatic extension of the brain slice lifespan. Brain slice viability was validated through electrophysiological recordings as well as live/dead cell assays. This system benefits researchers by monitoring incubation conditions and standardizing this artificial environment. It further provides viable tissue for two experimental days, reducing the time spent preparing brain slices and the number of animals required for research.
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影响因子：
2.5
作者：
Buskila, Yossi;Amitai, Yael
通讯作者：
Amitai, Yael
影响因子：
5.5
作者：
GUTFREUND, Y;YAROM, Y;SEGEV, I
通讯作者：
SEGEV, I
影响因子：
4.1
作者：
MCILWAIN, H;BUCHEL, L;CHESHIRE, JD
通讯作者：
CHESHIRE, JD
影响因子：
3
作者：
COLLINGRIDGE, GL
通讯作者：
COLLINGRIDGE, GL
影响因子：
2.5
作者：
FUKUDA, A;CZURKO, A;NISHINO, H
通讯作者：
NISHINO, H