A high-strength mineralized collagen bone scaffold for large-sized cranial bone defect repair in sheep.
A high-strength mineralized collagen bone scaffold for large-sized cranial bone defect repair in sheep.
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用于修复绵羊大面积颅骨缺损的高强度矿化胶原骨支架
DOI：
10.1093/rb/rby020
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发表时间：
2018-10
影响因子：
6.7
通讯作者：
Zhang C
中科院分区：
文献类型：
--
作者：
Wang S;Zhao Z;Yang Y;Mikos AG;Qiu Z;Song T;Cui F;Wang X;Zhang C
摘要颅骨大面积缺损的修复是临床上的一大挑战。理想的颅骨修补材料必须具有足够的力学支撑、良好的生物相容性、良好的骨整合性和生物可降解性，才能实现颅骨缺损的功能性和美观性修复。本研究通过与聚甲基丙烯酸甲酯和钛网两种传统颅骨修补材料的比较，开发了一种具有仿生成分、微观结构和力学性能的高强度矿化胶原（MC）骨支架，用于绵羊颅骨大面积缺损的修复。致密的MC支架没有明显的孔结构，因此具有良好的机械性能。支架的强度和弹性模量远高于天然松质骨，略低于天然密质骨。体外细胞相容性评价结果表明，人骨髓间充质干细胞（hBMSC）在致密MC支架上具有良好的存活、贴壁和增殖能力，具有良好的生物相容性。建立成年绵羊颅骨缺损模型，评价这些颅骨修补材料修复颅骨缺损的性能。通过大体观察、计算机断层扫描以及组织学评估来研究结果。体内评价表明，致密MC支架具有显著的骨传导性，并通过促进骨再生与周围颅骨组织形成骨整合。我们的研究结果表明，紧凑的MC支架具有良好的潜力，为大规模的颅骨缺损修复。
Abstract Large-sized cranial bone defect repair presents a great challenge in the clinic. The ideal cranioplasty materials to realize the functional and cosmetic recovery of the defect must have sufficient mechanical support, excellent biocompatibility, good osseointegration and biodegradability as well. In this study, a high-strength mineralized collagen (MC) bone scaffold was developed with biomimetic composition, microstructure and mechanical properties for the repair of sheep large-sized cranial bone defects in comparison with two traditional cranioplasty materials, polymethyl methacrylate and titanium mesh. The compact MC scaffold showed no distinct pore structure and therefore possessed good mechanical properties. The strength and elastic modulus of the scaffold were much higher than those of natural cancellous bone and slightly lower than those of natural compact bone. In vitro cytocompatibility evaluation revealed that the human bone marrow mesenchymal stem cells (hBMSC) had good viability, attachment and proliferation on the compact MC scaffold indicating its excellent biocompatibility. An adult sheep cranial bone defect model was constructed to evaluate the performances of these cranioplasty materials in repairing the cranial bone defects. The results were investigated by gross observation, computed tomography scanning as well as histological assessments. The in vivo evaluations indicated that compact MC scaffold showed notable osteoconductivity and osseointegration with surrounding cranial bone tissues by promoting bone regeneration. Our results suggested that the compact MC scaffold has a promising potential for large-sized cranial bone defect repair.
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影响因子：
4
作者：
Cheng, Zhen-Jiang;Wang, Xiu-Mei;Yan, Jian-Xin
通讯作者：
Yan, Jian-Xin
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影响因子：
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作者：
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通讯作者：
Cui F
影响因子：
14
作者：
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通讯作者：
Xu, Hockin H. K.
影响因子：
4.9
作者：
Li, Xiaoming;Huang, Yan;Fan, Yubo
通讯作者：
Fan, Yubo
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2
作者：
Chibbaro, Salvatore;Di Rocco, Fedreico;Bresson, Damien
通讯作者：
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