Umbilical cord and bone marrow mesenchymal stem cell seeding on macroporous calcium phosphate for bone regeneration in rat cranial defects.
Umbilical cord and bone marrow mesenchymal stem cell seeding on macroporous calcium phosphate for bone regeneration in rat cranial defects.
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DOI：
10.1016/j.biomaterials.2013.09.002
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发表时间：
2013-12
期刊：
影响因子：
14
通讯作者：
Xu, Hockin H. K.
中科院分区：
文献类型：
--
作者：
Chen, Wenchuan;Liu, Jun;Manuchehrabadi, Navid;Weir, Michael D.;Zhu, Zhimin;Xu, Hockin H. K.
关键词：
人脐带间充质干细胞(HUCMSCs)取材无穷无尽，无需侵入性操作即可低成本获取。然而，目前还没有将人骨髓间充质干细胞与人骨髓间充质干细胞体内成骨能力进行比较的报道。本研究的目的是研究hUCMSC和hBMSC在大孔磷酸钙骨水泥(CPC)上的种植情况，并比较它们在大鼠临界大小颅骨缺损中的骨再生情况。体外研究RGD修饰的大孔CPC的细胞贴壁、成骨分化和矿物质合成情况。将含有细胞的支架植入裸鼠8 mm的缺损处。术后4周、12周、24周分别行显微CT和组织学检查，观察骨再生情况。实验分为三组：植入hUCMSCs的CPC、植入hBMSCs的CPC和不含细胞的CPC对照组。体外培养的人脐血间充质干细胞和人骨髓间充质干细胞在CPC上的活细胞率和细胞密度相似。两种细胞均高表达碱性磷酸酶、骨钙素、I型胶原和Runx2。体内hUCMSC组和hBMSC组的骨密度和骨小梁厚度均大于CPC对照组。24周时，hUCMSC-CPC和hBMSC-CPC组的新骨量分别比CPC对照组增加57%和88%，血管密度分别增加15%和20%。HUCMSC-CPC组和hBMSC-CPC组的骨密度、新生骨量和血管密度基本相似。综上所述，首次发现种植在CPC上的hUCMSCs具有与hBMSCs体内相同的骨再生能力。HUCMSC-CPC和hBMSC-CPC构建的新骨和血管均比不含细胞的CPC多得多。大孔RGD复合干细胞移植的CPC在颅面及骨科修复中具有良好的应用前景。
Human umbilical cord mesenchymal stem cells (hUCMSCs) are inexhaustible and can be harvested at a low cost without an invasive procedure. However, there has been no report on comparing hUCMSCs with human bone marrow MSCs (hBMSCs) for bone regeneration in vivo. The aim of this study was to investigate hUCMSC and hBMSC seeding on macroporous calcium phosphate cement (CPC), and to compare their bone regeneration in critical-sized cranial defects in rats. Cell attachment, osteogenic differentiation and mineral synthesis on RGD-modified macroporous CPC were investigated in vitro. Scaffolds with cells were implanted in 8-mm defects of athymic rats. Bone regeneration was investigated via micro-CT and histological analysis at 4, 12, and 24 weeks. Three groups were tested: CPC with hUCMSCs, CPC with hBMSCs, and CPC control without cells. Percentage of live cells and cell density on CPC in vitro were similarly good for hUCMSCs and hBMSCs. Both cells had high osteogenic expressions of alkaline phosphatase, osteocalcin, collagen I, and Runx2. Bone mineral density and trabecular thickness in hUCMSC and hBMSC groups in vivo were greater than those of CPC control group. New bone amount for hUCMSC-CPC and hBMSC-CPC constructs was increased by 57% and 88%, respectively, while blood vessel density was increased by 15% and 20%, than CPC control group at 24 weeks. hUCMSC-CPC and hBMSC-CPC groups generally had statistically similar bone mineral density, new bone amount and vessel density. In conclusion, hUCMSCs seeded on CPC were shown to match the bone regeneration efficacy of hBMSCs in vivo for the first time. Both hUCMSC-CPC and hBMSC-CPC constructs generated much more new bone and blood vessels than CPC without cells. Macroporous RGD-grafted CPC with stem cell seeding is promising for craniofacial and orthopedic repairs.
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