Tangled1: a microtubule binding protein required for the spatial control of cytokinesis in maize.

Tangled1: a microtubule binding protein required for the spatial control of cytokinesis in maize.
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DOI:
10.1083/jcb.152.1.231
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发表时间:
2001-01-08
期刊:
The Journal of cell biology
影响因子:
--
通讯作者:
Levy J
Levy J
中科院分区:
其他
文献类型:
--
作者:
Smith LG;Gerttula SM;Han S;Levy J

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植物细胞胞质分裂的空间控制依赖于细胞动力学装置,即片质体,在有丝分裂前到达皮质“分裂位点”。以前,我们发现玉米的Tangled1 (Tan1)基因在玉米叶片发育过程中是必需的(Cleary, a.l., and L.G. Smith. 1998)。植物细胞。10:1875-1888。在这里,我们发现Tan1基因在分裂细胞中表达,并编码一种可以直接结合微管(MTs)的高度碱性蛋白。此外,抗tan1抗体识别的蛋白质优先与含有mt的细胞骨架结构相关,这些结构在tan1突变体的分裂细胞中定向错误。这些结果表明,TAN1蛋白通过与MTs的关联参与细胞分裂过程中细胞骨架结构的定向。
Spatial control of cytokinesis in plant cells depends on guidance of the cytokinetic apparatus, the phragmoplast, to a cortical “division site” established before mitosis. Previously, we showed that the Tangled1 (Tan1) gene of maize is required for this process during maize leaf development (Cleary, A.L., and L.G. Smith. 1998. Plant Cell. 10:1875–1888.). Here, we show that the Tan1 gene is expressed in dividing cells and encodes a highly basic protein that can directly bind to microtubules (MTs). Moreover, proteins recognized by anti-TAN1 antibodies are preferentially associated with the MT-containing cytoskeletal structures that are misoriented in dividing cells of tan1 mutants. These results suggest that TAN1 protein participates in the orientation of cytoskeletal structures in dividing cells through an association with MTs.
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