Distinct branches of the N-end rule pathway modulate the plant immune response.

Distinct branches of the N-end rule pathway modulate the plant immune response.
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N 端规则途径的不同分支调节植物免疫反应。

DOI:
10.1111/nph.15387
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发表时间:
2019
期刊:
The New phytologist
影响因子:
--
通讯作者:
Vicente J
Vicente J
中科院分区:
--
文献类型:
--
作者:
Vicente J

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N端规则途径是泛素蛋白酶体系统中高度保守的组成部分,但对其生物学作用知之甚少。本文探讨了N端规则途径在植物免疫反应中的作用。我们研究了该途径的组成部分和已知蛋白质底物对病原体感染的生理、生化和代谢反应的遗传影响。我们通过 E3 连接酶蛋白水解 (PRT)6 表明,谷氨酰胺 (Gln) 脱酰胺和半胱氨酸 (Cys) 氧化分支都是植物免疫系统的组成部分。在拟南芥中,Gln 特异性氨基末端 (Nt) 酰胺酶 (NTAQ1) 控制特定防御反应基因的表达,激活植物抗毒素 camalexin 的合成途径,并影响对半生物营养型病原体丁香假单胞菌番茄 (Pst) 的基础抗性。 Nt-Cys 乙烯反应因子 VII 转录因子底物增强病原体诱导的气孔关闭。 HvPRT6 表达减少的转基因大麦表现出对 Ps 的抗性增强。粳稻和 Blumeria graminisf。 sp.hordei,表明该途径的保守作用。我们提出,N-末端规则途径的单独分支充当开花植物中植物免疫反应的不同组成部分。
The N‐end rule pathway is a highly conserved constituent of the ubiquitin proteasome system, yet little is known about its biological roles.Here we explored the role of the N‐end rule pathway in the plant immune response. We investigated the genetic influences of components of the pathway and known protein substrates on physiological, biochemical and metabolic responses to pathogen infection.We show that the glutamine (Gln) deamidation and cysteine (Cys) oxidation branches are both components of the plant immune system, through the E3 ligase PROTEOLYSIS (PRT)6. InArabidopsis thalianaGln‐specific amino‐terminal (Nt)‐amidase (NTAQ1) controls the expression of specific defence‐response genes, activates the synthesis pathway for the phytoalexin camalexin and influences basal resistance to the hemibiotroph pathogenPseudomonas syringaepvtomato(Pst). The Nt‐Cys ETHYLENE RESPONSE FACTOR VII transcription factor substrates enhance pathogen‐induced stomatal closure. Transgenic barley with reducedHvPRT6expression showed enhanced resistance toPs. japonicaandBlumeria graminisf. sp.hordei, indicating a conserved role of the pathway.We propose that that separate branches of the N‐end rule pathway act as distinct components of the plant immune response in flowering plants.
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