Genome-Wide Profiling of Plutella xylostella Immunity-Related miRNAs after Isaria fumosorosea Infection.
Genome-Wide Profiling of Plutella xylostella Immunity-Related miRNAs after Isaria fumosorosea Infection.
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小菜蛾感染后免疫相关 miRNA 的全基因组分析
DOI：
10.3389/fphys.2017.01054
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发表时间：
2017
影响因子：
4
通讯作者：
Jin F
中科院分区：
文献类型：
--
作者：
Xu J;Xu X;Li S;Wang S;Xu X;Zhou X;Yu J;Yu X;Shakeel M;Jin F
小菜蛾对几乎所有杀虫剂的抗药性发展在世界范围内受到了广泛关注。昆虫病原真菌如玫烟色棒束孢已被用作杀虫剂的替代品。然而，对miRNA调控的抗昆虫病原真菌反应的认识仍处于婴儿阶段。在本研究中，小菜蛾受到I.在包括对照的四个不同时间点（12、18、24和36 h），用含玫烟色孢的质粒构建miRNA文库，通过Illumina测序构建miRNA文库。差异表达分析结果显示，与对照组相比，所有处理组中有23个miRNAs差异表达。值得一提的是，其中，一些保守的miRNA，如miR-2，miR-9a，miR-745，miR-7 b和miR-2767，已知在宿主-病原体相互作用中发挥关键作用，也被鉴定出来。此外，通过RT-qPCR验证差异表达的miRNA。这些结果为进一步研究I. fumosorosea和P. xylostella在转录后水平的表达。
The development of resistance by Plutella xylostella to almost all insecticides is of significant concern all over the world. Entomopathogenic fungi such as Isaria fumosorosea have been used as an alternative to insecticides. However, the knowledge of miRNA-regulated reactions against entomopathogenic fungi is still in its infant stage. In the present study, P. xylostella was challenged with I. fumosorosea at four different time points (12, 18, 24, and 36 h) including a control, to build miRNA libraries by Illumina sequencing. The results of differential expression analysis exhibited that 23 miRNAs were differentially expressed, compared to control, in all treatments. It is worth mentioning, of these, some conserved miRNAs such as miR-2, miR-9a, miR-745, miR-7b, and miR-2767, known to play critical roles in host-pathogen interaction, were also identified. Furthermore, differentially expressed miRNAs were validated by RT-qPCR. Our results provide an essential information for further functional studies of the interaction between I. fumosorosea and P. xylostella at the post-transcriptional level.
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影响因子：
48
作者：
Langmead, Ben;Salzberg, Steven L.
通讯作者：
Salzberg, Steven L.
影响因子：
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作者：
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通讯作者：
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通讯作者：
Vilcinskas A
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作者：
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通讯作者：
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影响因子：
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作者：
Imler, Jean-Luc
通讯作者：
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