Cerebral dopamine neurotrophic factor promotes the proliferation and differentiation of neural stem cells in hypoxic environments.
Cerebral dopamine neurotrophic factor promotes the proliferation and differentiation of neural stem cells in hypoxic environments.
复制标题
脑多巴胺神经营养因子促进缺氧环境下神经干细胞的增殖和分化
DOI：
10.4103/1673-5374.282262
复制
发表时间：
2020-11
影响因子：
6.1
通讯作者：
Chen LK
中科院分区：
文献类型：
--
作者：
Lin CQ;Chen LK
Previous research found that cerebral dopamine neurotrophic factor (CDNF) has a protective effect on brain dopaminergic neurons, and CDNF is regarded as a promising therapeutic agent for neurodegenerative diseases. However, the effects of CDNF on the proliferation, differentiation, and apoptosis of neural stem cells (NSCs), which are very sensitive to hypoxic environments, remain unknown. In this study, NSCs were extracted from the hippocampi of fetal rats and cultured with different concentrations of CDNF. The results showed that 200 nM CDNF was the optimal concentration for significantly increasing the viability of NSCs under non-hypoxic environmental conditions. Then, the cells were cultured with 200 nM CDNF under the hypoxic conditions of 90% N2, 5% CO2, and 5% air for 6 hours. The results showed that CDNF significantly improved the viability of hypoxic NSCs and reduced apoptosis among hypoxic NSCs. The detection of markers showed that CDNF increased the differentiation of hypoxic NSCs into neurons and astrocytes. CDNF also reduced the expression level of Lin28 protein and increased the expression of Let-7 mRNA in NSCs, under hypoxic conditions. In conclusion, we determined that CDNF was able to reverse the adverse proliferation, differentiation, and apoptosis effects that normally affect NSCs in a hypoxic environment. Furthermore, the Lin28/Let-7 pathway may be involved in this regulated function of CDNF. The present study was approved by the Laboratory Animal Centre of Southeast University, China (approval No. 20180924006) on September 24, 2018.
登录
查看更多内容
影响因子：
2.9
作者：
Hayashi, T;Iwai, M;Abe, K
通讯作者：
Abe, K
DOI：
10.1016/j.pnpbp.2016.04.009
发表时间：
2016-08-01
影响因子：
5.6
作者：
Lee, Min Young;Kim, Eun Young;Ahn, Yong Min
通讯作者：
Ahn, Yong Min
影响因子：
3.3
作者：
Mei, Jia-ming;Niu, Chao-shi
通讯作者：
Niu, Chao-shi
影响因子：
3.3
作者：
McGrath, Erica L.;Gao, Junling;Wu, Ping
通讯作者：
Wu, Ping
影响因子：
3.3
作者：
Gao J;Thonhoff JR;Dunn TJ;Wu P
通讯作者：
Wu P