Transcriptome profiles of the protoscoleces of Echinococcus granulosus reveal that excretory-secretory products are essential to metabolic adaptation.
Transcriptome profiles of the protoscoleces of Echinococcus granulosus reveal that excretory-secretory products are essential to metabolic adaptation.
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细粒棘球绦虫原头节的转录组谱揭示排泄分泌产物对于代谢适应至关重要
DOI：
10.1371/journal.pntd.0003392
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发表时间：
2014-12
影响因子：
3.8
通讯作者：
Cao J
中科院分区：
文献类型：
--
作者：
Pan W;Shen Y;Han X;Wang Y;Liu H;Jiang Y;Zhang Y;Wang Y;Xu Y;Cao J
囊状棘球蚴病（CHD）是由绦虫的幼虫阶段引起的，在世界范围内影响人类和家畜。原寄生虫（PSC）是幼虫阶段的一个组成部分，可以与终末宿主和中间宿主相互作用。以前的基因组和转录组数据提供了这种寄生虫的生长和发育的基因组学的整体快照。然而，我们对PSC如何破坏宿主的免疫反应并维持代谢适应的理解仍然不清楚。在本研究中，我们使用Roche 454测序技术和电子分泌组分析来探索E.的颗粒和阐明的排泄分泌蛋白（ESP）释放的寄生虫的潜在功能。方法/主要发现产生了大量的非冗余序列作为单基因（26，514），其中22，910（86.4%）被定位到新发表的E. 17，705个（66.8%）分布在编码序列（CDS）区域。在从转录组预测的2，280个ESP中，138个ESP被推断参与碳水化合物的代谢，而124个ESP被推断参与蛋白质的代谢。11种ESP被鉴定为调节糖酵解/糖异生（GL/GN）途径的细胞内酶，而另外44种抗原蛋白、25种分子伴侣和4种蛋白酶被高度代表。还发现许多蛋白质在发育相关的信号通路中显著富集，例如TGF-β受体通路和胰岛素通路。结论/意义-本研究提供了有价值的信息代谢适应的寄生虫，其主机，可用于帮助开发新的干预目标包虫病的治疗和控制。
Background Cystic hydatid disease (CHD) is caused by the larval stages of the cestode and affects humans and domestic animals worldwide. Protoscoleces (PSCs) are one component of the larval stages that can interact with both definitive and intermediate hosts. Previous genomic and transcriptomic data have provided an overall snapshot of the genomics of the growth and development of this parasite. However, our understanding of how PSCs subvert the immune response of hosts and maintains metabolic adaptation remains unclear. In this study, we used Roche 454 sequencing technology and in silico secretome analysis to explore the transcriptome profiles of the PSCs from E. granulosus and elucidate the potential functions of the excretory-secretory proteins (ESPs) released by the parasite. Methodology/Principal Findings A large number of nonredundant sequences as unigenes were generated (26,514), of which 22,910 (86.4%) were mapped to the newly published E. granulosus genome and 17,705 (66.8%) were distributed within the coding sequence (CDS) regions. Of the 2,280 ESPs predicted from the transcriptome, 138 ESPs were inferred to be involved in the metabolism of carbohydrates, while 124 ESPs were inferred to be involved in the metabolism of protein. Eleven ESPs were identified as intracellular enzymes that regulate glycolysis/gluconeogenesis (GL/GN) pathways, while a further 44 antigenic proteins, 25 molecular chaperones and four proteases were highly represented. Many proteins were also found to be significantly enriched in development-related signaling pathways, such as the TGF-β receptor pathways and insulin pathways. Conclusions/Significance This study provides valuable information on the metabolic adaptation of parasites to their hosts that can be used to aid the development of novel intervention targets for hydatid treatment and control.
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影响因子：
3.9
作者：
Andrade, MA;Siles-Lucas, M;Muro, A
通讯作者：
Muro, A
影响因子：
2.4
作者：
Craig, H;Wastling, JM;Knox, DP
通讯作者：
Knox, DP
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1.5
作者：
Crook, M;Thompson, FJ;Viney, ME
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Viney, ME
影响因子：
2.4
作者：
Carmena, D;Martínez, J;Guisantes, JA
通讯作者：
Guisantes, JA
影响因子：
4.4
作者：
Garg G;Ranganathan S
通讯作者：
Ranganathan S