A culture system to study oligodendrocyte myelination processes using engineered nanofibers.
A culture system to study oligodendrocyte myelination processes using engineered nanofibers.
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DOI：
10.1038/nmeth.2105
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发表时间：
2012-09
期刊：
影响因子：
48
通讯作者：
Chan, Jonah R.
中科院分区：
文献类型：
--
作者：
Lee, Seonok;Leach, Michelle K.;Redmond, Stephanie A.;Chong, S. Y. Christin;Mellon, Synthia H.;Tuck, Samuel J.;Feng, Zhang-Qi;Corey, Joseph M.;Chan, Jonah R.
目前研究中枢神经系统髓鞘形成的方法需要有利于少突胶质细胞包裹髓鞘的允许轴突基质。我们已经开发了一种无神经元的培养系统，其中不同大小的电子自旋纳米纤维替代轴突作为少突胶质细胞髓鞘形成的底物，从而允许操纵轴突-少突胶质细胞相互作用的生物物理元素。为了研究髓鞘形成的轴突调节，该系统有效地将分子（诱导）线索的作用与轴突的生物物理性质的作用分离。我们用这种方法来揭示纤维直径在大鼠少突胶质细胞同心缠绕起始中的原因和充分性。我们还表明，少突胶质细胞前体细胞显示纤维直径的生物物理特性的敏感性，并在分化前启动膜鞘。使用神经再生支架将能够筛选促进少突胶质细胞分化和髓鞘形成的潜在治疗剂，并为髓鞘再生过程提供有价值的见解。
Current methods for studying central nervous system myelination necessitate permissive axonal substrates conducive for myelin wrapping by oligodendrocytes. We have developed a neuron-free culture system in which electron-spun nanofibers of varying sizes substitute for axons as a substrate for oligodendrocyte myelination, thereby allowing manipulation of the biophysical elements of axonal-oligodendroglial interactions. To investigate axonal regulation of myelination, this system effectively uncouples the role of molecular (inductive) cues from that of biophysical properties of the axon. We use this method to uncover the causation and sufficiency of fiber diameter in the initiation of concentric wrapping by rat oligodendrocytes. We also show that oligodendrocyte precursor cells display sensitivity to the biophysical properties of fiber diameter and initiate membrane ensheathment prior to differentiation. The use of nanofiber scaffolds will enable screening for potential therapeutic agents that promote oligodendrocyte differentiation and myelination as well as provide valuable insight into the processes involved in remyelination.
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影响因子：
4.4
作者：
REMAHL, S;HILDEBRAND, C
通讯作者：
HILDEBRAND, C
影响因子：
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Leach, Michelle K.;Feng, Zhang-Qi;Corey, Joseph M.
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作者：
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影响因子：
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作者：
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通讯作者：
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