Handheld Microfluidic Filtration Platform Enables Rapid, Low-Cost, and Robust Self-Testing of SARS-CoV-2 Virus.
Handheld Microfluidic Filtration Platform Enables Rapid, Low-Cost, and Robust Self-Testing of SARS-CoV-2 Virus.
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DOI：
10.1002/smll.202104009
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发表时间：
2021-12
期刊：
影响因子：
--
通讯作者：
Lao T
中科院分区：
文献类型：
--
作者：
Xu J;Suo W;Goulev Y;Sun L;Kerr L;Paulsson J;Zhang Y;Lao T
新冠肺炎疫情引发了前所未有的公共卫生和经济危机。防止进一步传播在很大程度上取决于对感染者的及时和准确识别。在这里，我们报告了一种新型的微流控检测试剂盒，它结合了超高通量流体动力过滤和夹心免疫分析。具体地说，包被两种不同的非竞争性抗体的纳米和微珠被用来同时捕获SARS-CoV-2核衣壳(N)蛋白，形成更大的复合体。微流控过滤通过规定宽度的柱缝丢弃自由纳米棒，但在观察区保留抗原桥联的复合体，在观察区显示红色表示样品中存在抗原。这个测试平台表现出高通量分离(<；30秒)和抗原浓缩，超过了传统的侧向流动分析或微流控分析，检测下限(LOD)<；100拷贝/毫升。在2020年12月(93人)和2021年8月(12人)进行的两轮临床试验中，我们的检测显示出高灵敏度，分别为95.4%和100%，证明该微流控检测试剂盒能够检测SARS-CoV-2病毒变异。此外，批量生产的芯片可以以每个测试0.98美元的成本制造，并且坚固的设计允许芯片重复使用50次以上。所有这些特点使我们的微流控检测试剂盒特别适合医疗基础设施不足和实验室资源短缺的地区。提出了一种用于SARS-CoV-2病毒检测的夹心免疫分析和流体力学过滤相结合的微流控诊断平台。纳米和微珠被高亲和力抗体包裹，以捕获SARS-CoV-2核衣壳蛋白。微流控过滤通过30μm宽的柱缝丢弃游离纳米棒，但在观察区保留抗原桥联的复合体，在观察区显示红色表示样品中存在抗原。
The COVID-19 pandemic has led to an unprecedented crisis of both public health and economy. The prevention of further spread relies greatly on the timely and accurate identification of infected individuals. Here, we report of a novel microfluidic test kit combining ultrahigh throughput hydrodynamic filtration and sandwich immunoassay. Specifically, nano and micro- beads coated with two different, noncompetitive antibodies, are used to capture SARS-CoV-2 nucleocapsid (N) proteins simultaneously, forming larger complexes. Microfluidic filtration discards free nanobeads through pillar gaps with a defined width but retains antigen-bridged complexes in the observation zone, where a display of red color indicates the presence of antigen in the sample. This testing platform exhibits high throughput separation (<30 seconds) and enrichment of antigen that exceeds the traditional lateral flow assays or microfluidic assays, with a low limit of detection (LoD) < 100 copies/mL. In two rounds of clinical trials conducted in December 2020 (93 individuals) and August 2021 (12 individuals), our assays demonstrate high sensitivities of 95.4% and 100%, respectively, which proves this microfluidic test kit capable of detecting SARS-CoV-2 virus variants evolved over significant periods of time. Furthermore, the mass-produced chip can be fabricated at a cost of $0.98/test and the robust design allows the chip to be reused for over 50 times. All of these features make our microfluidic test kit particularly suitable for areas with inadequate medical infrastructure and a shortage of laboratory resources. A microfluidic diagnostic platform combining sandwich immunoassay and hydrodynamic filtration is presented for detection of SARS-CoV-2 virus. The nano- and micro- beads are coated with high-affinity antibodies to capture SARS-CoV-2 nucleocapsid proteins. Microfluidic filtration discards free nanobeads through pillar gaps with 30 μm in width but retains antigen-bridged complexes in the observation zone, where a display of red color indicates the presence of antigen in the sample.
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