Magnetic Levitation of MC3T3 Osteoblast Cells as a Ground-Based Simulation of Microgravity.
Magnetic Levitation of MC3T3 Osteoblast Cells as a Ground-Based Simulation of Microgravity.
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DOI：
10.1007/s12217-008-9092-6
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发表时间：
2009-11
影响因子：
1.8
通讯作者：
Xu, Wayne Wenzhong
中科院分区：
文献类型：
--
作者：
Hammer, Bruce E.;Kidder, Louis S.;Williams, Philip C.;Xu, Wayne Wenzhong
放置在强磁场和磁场梯度中的抗磁性样品会受到磁力。稳定的磁悬浮发生时，磁力正好抵消重力。在此条件下，抗磁性样品处于模拟微重力环境中。本研究的目的是探索MC 3 T3-E1成骨细胞是否可以在磁模拟的hypo-g和hyper-g环境中生长，并确定在这些条件下基因表达是否差异表达。将在Cytodex-3珠上生长的鼠颅骨成骨细胞系MC 3 T3-E1在17 T超导磁体中经受0、1和2 g的净重力2天。对这些细胞的微阵列分析表明，重力应激导致数百个基因的上调和下调。讨论了维持生物系统长期磁悬浮的方法。
Diamagnetic samples placed in a strong magnetic field and a magnetic field gradient experience a magnetic force. Stable magnetic levitation occurs when the magnetic force exactly counter balances the gravitational force. Under this condition, a diamagnetic sample is in a simulated microgravity environment. The purpose of this study is to explore if MC3T3-E1 osteoblastic cells can be grown in magnetically simulated hypo-g and hyper-g environments and determine if gene expression is differentially expressed under these conditions. The murine calvarial osteoblastic cell line, MC3T3-E1, grown on Cytodex-3 beads, were subjected to a net gravitational force of 0, 1 and 2 g in a 17 T superconducting magnet for 2 days. Microarray analysis of these cells indicated that gravitational stress leads to up and down regulation of hundreds of genes. The methodology of sustaining long-term magnetic levitation of biological systems are discussed.
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影响因子：
4.1
作者：
Harris, SA;Zhang, M;Turner, RT
通讯作者：
Turner, RT
影响因子：
3.8
作者：
Coleman, Chasity B.;Gonzalez-Villalobos, Romer A.;Hammond, Timothy G.
通讯作者：
Hammond, Timothy G.
DOI：
10.1083/jcb.96.1.191
发表时间：
1983-01
期刊：
The Journal of cell biology
影响因子：
--
作者：
Sudo H;Kodama HA;Amagai Y;Yamamoto S;Kasai S
通讯作者：
Kasai S
影响因子：
4
作者：
Guevorkian, K;Valles, JM
通讯作者：
Valles, JM
影响因子：
3.2
作者：
UENO, S;IWASAKA, M
通讯作者：
IWASAKA, M