In vitro differentiation and calcification in a new clonal osteogenic cell line derived from newborn mouse calvaria.
In vitro differentiation and calcification in a new clonal osteogenic cell line derived from newborn mouse calvaria.
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DOI：
10.1083/jcb.96.1.191
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发表时间：
1983-01
期刊：
影响因子：
--
通讯作者：
Kasai S
中科院分区：
文献类型：
--
作者：
Sudo H;Kodama HA;Amagai Y;Yamamoto S;Kasai S
我们研究了克隆成骨细胞系MC 3 T3-E1的能力，建立从新生小鼠颅骨和选择的基础上，高碱性磷酸酶（ALP）活性的融合状态，分化成骨细胞和矿化在体外。处于生长状态的细胞呈成纤维细胞形态，并生长形成多层。在第21天，在嗜锇结节区域发现具有典型成骨细胞形态的细胞簇。这种结节的数量和大小随着培养时间的增加而增加，到第40-50天，肉眼就很容易识别。在发育良好的结节的中央部分，骨细胞嵌入高度矿化的骨基质中。成骨细胞排列在骨针周围，周围有富含溶酶体的细胞和成纤维细胞层。成骨细胞和年轻骨细胞周围散在大量基质囊泡。成骨细胞、年轻骨细胞和富含溶酶体的细胞的基质囊泡和质膜对ALP活性和钙离子的细胞化学染色表现出强烈的反应。矿物质最初定位于基质囊泡中，然后沉积在带状胶原纤维上。沉积的矿物质仅由钙和磷组成，其中一些晶体已成熟为羟基磷灰石晶体。这些结果表明，MC 3 T3-E1细胞在体外具有分化为成骨细胞和骨细胞以及形成钙化骨组织的能力。
We investigated the capacity of a clonal osteogenic cell line MC3T3-E1, established from newborn mouse calvaria and selected on the basis of high alkaline phosphatase (ALP) activity in the confluent state, to differentiate into osteoblasts and mineralize in vitro. The cells in the growing state showed a fibroblastic morphology and grew to form multiple layers. On day 21, clusters of cells exhibiting typical osteoblastic morphology were found in osmiophilic nodular regions. Such nodules increased in number and size with incubation time and became easily identifiable with the naked eye by day 40-50. In the central part of well-developed nodules, osteocytes were embedded in heavily mineralized bone matrix. Osteoblasts were arranged at the periphery of the bone spicules and were surrounded by lysosome-rich cells and a fibroblastic cell layer. Numerous matrix vesicles were scattered around the osteoblasts and young osteocytes. Matrix vesicles and plasma membranes of osteoblasts, young osteocytes, and lysosome-rich cells showed strong reaction to cytochemical stainings for ALP activity and calcium ions. Minerals were initially localized in the matrix vesicles and then deposited on well-banded collagen fibrils. Deposited minerals consisted exclusively of calcium and phosphorus, and some of the crystals had matured into hydroxyapatite crystals. These results indicate that MC3T3-E1 cells have the capacity to differentiate into osteoblasts and osteocytes and to form calcified bone tissue in vitro.
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DOI：
10.1111/j.1365-2818.1975.tb03895.x
发表时间：
1975-01-01
期刊：
JOURNAL OF MICROSCOPY-OXFORD
影响因子：
--
作者：
CLIFF, G;LORIMER, GW
通讯作者：
LORIMER, GW
DOI：
10.1083/jcb.14.3.357
发表时间：
1962-09
期刊：
The Journal of cell biology
影响因子：
--
作者：
YOUNG RW
通讯作者：
YOUNG RW
DOI：
10.1007/bf00326615
发表时间：
1967-01-01
期刊：
HISTOCHEMIE
影响因子：
--
作者：
MAYAHARA, H;HIRANO, H;OGAWA, K
通讯作者：
OGAWA, K
影响因子：
7.8
作者：
AUBIN, JE;HEERSCHE, JNM;SODEK, J
通讯作者：
SODEK, J
DOI：
10.1111/j.1749-6632.1960.tb49972.x
发表时间：
1960-01-01
影响因子：
5.2
作者：
BURSTONE, MS
通讯作者：
BURSTONE, MS