Bone response to fluoride exposure is influenced by genetics.
Bone response to fluoride exposure is influenced by genetics.
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骨对氟化物暴露的反应受遗传学的影响。
DOI：
10.1371/journal.pone.0114343
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发表时间：
2014
期刊：
影响因子：
3.7
通讯作者：
Buzalaf MA
中科院分区：
文献类型：
--
作者：
Kobayashi CA;Leite AL;Peres-Buzalaf C;Carvalho JG;Whitford GM;Everett ET;Siqueira WL;Buzalaf MA
遗传因素影响氟对釉质形成和骨稳态的影响，但其潜在的分子机制尚不清楚。采用无标记蛋白质组学方法鉴定和评价两种小鼠品系中骨蛋白表达的变化，这两种小鼠品系具有不同的发生氟斑牙和改变骨质量的可能性。在体内骨形成和组织形态计量学F摄入后，也进行了评估和相关的蛋白质组。将抗性129 P3/J和易感A/J小鼠分为三组，给予低F食物和含有0、10或50 ppmF的水8周。评价血浆的碱性磷酸酶活性。使用显微CT分析评估股骨、胫骨和腰椎，并测量皮质骨中的矿物质沉积率（MAR）。对于定量蛋白质组学分析，提取骨蛋白，并使用液相色谱-电喷雾电离-串联质谱法（LC-ESI-MS/MS）进行分析，然后进行无标记半定量差异表达分析。在每个小鼠品系内的F处理组之间以及每个F处理组的品系之间发现了几种骨蛋白的变化（比值≥1.5或≤0.5; p<0.05）。虽然F处理对两种品系的BMD或骨组织形态计量学没有显著影响，但50 ppmF 129 P3/J小鼠的MAR高于用50 ppmF处理的50 ppmF A/J小鼠，表明F以品系特异性方式增加骨形成。此外，氟暴露与剂量特异性和应变特异性的蛋白质的表达参与成骨细胞和破骨细胞的变化。总之，我们的研究结果证实了遗传的影响，在骨响应氟暴露和点几种蛋白质，可能作为目标的差异F反应在这个组织。
Genetic factors influence the effects of fluoride (F) on amelogenesis and bone homeostasis but the underlying molecular mechanisms remain undefined. A label-free proteomics approach was employed to identify and evaluate changes in bone protein expression in two mouse strains having different susceptibilities to develop dental fluorosis and to alter bone quality. In vivo bone formation and histomorphometry after F intake were also evaluated and related to the proteome. Resistant 129P3/J and susceptible A/J mice were assigned to three groups given low-F food and water containing 0, 10 or 50 ppmF for 8 weeks. Plasma was evaluated for alkaline phosphatase activity. Femurs, tibiae and lumbar vertebrae were evaluated using micro-CT analysis and mineral apposition rate (MAR) was measured in cortical bone. For quantitative proteomic analysis, bone proteins were extracted and analyzed using liquid chromatography-electrospray ionization-tandem mass spectrometry (LC-ESI-MS/MS), followed by label-free semi-quantitative differential expression analysis. Alterations in several bone proteins were found among the F treatment groups within each mouse strain and between the strains for each F treatment group (ratio ≥1.5 or ≤0.5; p<0.05). Although F treatment had no significant effects on BMD or bone histomorphometry in either strain, MAR was higher in the 50 ppmF 129P3/J mice than in the 50 ppmF A/J mice treated with 50 ppmF showing that F increased bone formation in a strain-specific manner. Also, F exposure was associated with dose-specific and strain-specific alterations in expression of proteins involved in osteogenesis and osteoclastogenesis. In conclusion, our findings confirm a genetic influence in bone response to F exposure and point to several proteins that may act as targets for the differential F responses in this tissue.
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影响因子：
7.6
作者：
Everett, ET;McHenry, MAK;Stookey, GK
通讯作者：
Stookey, GK
DOI：
10.1126/science.1215063
发表时间：
2012-01-13
期刊：
Science (New York, N.Y.)
影响因子：
--
作者：
Baker JL;Sudarsan N;Weinberg Z;Roth A;Stockbridge RB;Breaker RR
通讯作者：
Breaker RR
影响因子：
4.1
作者：
Mousny, M.;Omelon, S.;Wise, L.;Everett, E. T.;Dumitriu, M.;Holmyard, D. P.;Banse, X.;Devogelaer, J. P.;Grynpas, Marc D.
通讯作者：
Grynpas, Marc D.
影响因子：
4
作者：
Balena, R;Kleerekoper, M;Parfitt, AM
通讯作者：
Parfitt, AM
影响因子：
64.5
作者：
Komori, T;Yagi, H;Kishimoto, T
通讯作者：
Kishimoto, T