Fluoride effects on bone formation and mineralization are influenced by genetics.
Fluoride effects on bone formation and mineralization are influenced by genetics.
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DOI：
10.1016/j.bone.2008.07.248
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发表时间：
2008-12
期刊：
影响因子：
4.1
通讯作者：
Grynpas, Marc D.
中科院分区：
文献类型：
--
作者：
Mousny, M.;Omelon, S.;Wise, L.;Everett, E. T.;Dumitriu, M.;Holmyard, D. P.;Banse, X.;Devogelaer, J. P.;Grynpas, Marc D.
关键词：
骨对氟化物（F−）暴露的反应变化归因于遗传因素。对三种不同氟斑牙易感性的近交系小鼠（A/J、SWR/J、129 P3/J），增加氟剂量（0 ppm、25 ppm、50 ppm、100 ppm），其皮质骨和松质骨的力学性能受到不同程度的影响。在本文中，骨的结构和材料特性进行了评价，以解释以前观察到的力学性能的变化。本研究评估了增加氟剂量对A/J、SWR/J和129 P3/J小鼠品系的骨形成、微结构、矿化和显微硬度的影响。骨微结构定量与微计算机断层扫描和支柱分析。通过静态组织形态计量学评价骨形成。骨矿化定量背散射电子（BSE）成像和粉末X射线衍射。显微硬度测量取自椎体（皮质骨和松质骨）和股骨远端皮质。氟处理对任何菌株的骨微结构没有显着影响。所有三种菌株都表现出在最大氟化物剂量下类骨质形成的显著增加。椎体松质骨BSE成像显示，在50 ppm和100 ppm F−下，SWR/J菌株的矿化异质性显著降低。在三种菌株中，随着F−剂量的增加，骨小梁和皮质骨矿化特征显示出向更高矿化的非显著性转变。粉末X射线衍射显示129 P3/J菌株的晶体明显更小，并且所有菌株的晶体宽度随着F−剂量的增加而增加。F−对骨小梁和皮质骨显微硬度没有影响。氟处理对这三个品系的骨微结构没有显著影响。类骨质形成增加和矿化异质性降低支持F−延迟新骨矿化的理论。随着F-剂量的增加，晶体宽度增加，证实了早期的结果，并与大多数机械性能下降相关。骨F-的增加可能会影响矿物-有机界面结合和/或骨基质蛋白，干扰微晶表面上的骨晶体生长抑制以及矿物和有机界面之间的结合。129 P3/J（抗性）菌株的较小骨微晶可能表明更强的有机/无机界面，降低微晶生长速率并增加界面机械强度。
A variation in bone response to fluoride (F−) exposure has been attributed to genetic factors. Increasing fluoride doses (0ppm, 25ppm, 50ppm, 100ppm) for three inbred mouse strains with different susceptibilities to developing dental enamel fluorosis (A/J, a “susceptible” strain; SWR/J, an “intermediate” strain; 129P3/J, a “resistant” strain) had different effects on their cortical and trabecular bone mechanical properties. In this paper, the structural and material properties of the bone were evaluated to explain the previously observed changes in mechanical properties. This study assessed the effect of increasing fluoride doses on the bone formation, microarchitecture, mineralization and microhardness of the A/J, SWR/J and 129P3/J mouse strains. Bone microarchitecture was quantified with microcomputed tomography and strut analysis. Bone formation was evaluated by static histomorphometry. Bone mineralization was quantified with backscattered electron (BSE) imaging and powder x-ray diffraction. Microhardness measurements were taken from the vertebral bodies (cortical and trabecular bone) and the cortex of the distal femur. Fluoride treatment had no significant effect on bone microarchitecture for any of the strains. All three strains demonstrated a significant increase in osteoid formation at the largest fluoride dose. Vertebral body trabecular bone BSE imaging revealed significantly decreased mineralization heterogeneity in the SWR/J strain at 50ppm and 100ppm F−. The trabecular and cortical bone mineralization profiles showed a non-significant shift towards higher mineralization with increasing F− dose in the three strains. Powder x-ray diffraction showed significantly smaller crystals for the 129P3/J strain, and increased crystal width with increasing F− dose for all strains. There was no effect of F− on trabecular and cortical bone microhardness. Fluoride treatment had no significant effect on bone microarchitecture in these three strains. The increased osteoid formation and decreased mineralization heterogeneity support the theory that F− delays mineralization of new bone. The increasing crystal width with increasing F− dose confirms earlier results and correlates with most of the decreased mechanical properties. An increase in bone F− may affect the mineral-organic interfacial bonding and/or bone matrix proteins, interfering with bone crystal growth inhibition on the crystallite faces as well as bonding between the mineral and organic interface. The smaller bone crystallites of the 129P3/J (resistant) strain may indicate a stronger organic/inorganic interface, reducing crystallite growth rate and increasing interfacial mechanical strength.
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影响因子：
7.6
作者：
Everett, ET;McHenry, MAK;Stookey, GK
通讯作者：
Stookey, GK
影响因子：
4.1
作者：
COMPSTON, JE;GARRAHAN, NJ;YAMAGUCHI, K
通讯作者：
YAMAGUCHI, K
DOI：
10.1016/0221-8747(83)90016-4
发表时间：
1984-01-01
期刊：
METABOLIC BONE DISEASE & RELATED RESEARCH
影响因子：
--
作者：
BOYDE, A;JONES, SJ
通讯作者：
JONES, SJ
影响因子：
4.1
作者：
GRYNPAS, MD;REY, C
通讯作者：
REY, C
影响因子：
3.5
作者：
Kindt, J. H.;Thurner, P. J.;Hansma, P. K.
通讯作者：
Hansma, P. K.