Effects of electroacupuncture Zusanli (ST36) on food intake and expression of POMC and TRPV1 through afferents-medulla pathway in obese prone rats.
Effects of electroacupuncture Zusanli (ST36) on food intake and expression of POMC and TRPV1 through afferents-medulla pathway in obese prone rats.
复制标题
DOI：
10.1016/j.peptides.2012.10.009
复制
发表时间：
2013-02
期刊：
影响因子：
3
通讯作者：
Ma S
中科院分区：
文献类型：
--
作者：
Ji B;Hu J;Ma S
本研究的目的是确定电针（EA）ST 36对肥胖倾向（OP）大鼠的摄食量和体重的影响，并与肥胖抵抗（OR）品系的高脂饮食进行比较。电针对前阿黑皮素（POMC），瞬时受体电位香草酸1型（TRPV 1）和神经元型一氧化氮合酶（nNOS）的mRNA水平的影响也在延髓区和ST 36皮肤组织进行了检查。高级EA ST 36分两次进行，每次20分钟，间隔80分钟，持续7天。每天记录清醒大鼠的摄食量和体重。在处理结束时，在髓质区域和皮肤组织的微穿孔中进行真实的时间PCR。进一步电针ST 36可显著降低OP大鼠的摄食量和体重，但对OR品系和假电针大鼠的摄食量和体重略有降低。晚期EA ST 36在孤束核（NTS）和舌下神经核（HN）区域产生POMC mRNA水平的显著增加。电针可使OP大鼠NTS/薄束核区和ST 36皮肤区TRPV 1和nNOS mRNA表达同时增加。我们的结论是，先进的EA ST 36产生了上调的促氧化因子POMC生产的NTS/HN，这抑制了食物的摄入量，降低了体重。电针诱导的ST 36和NTS/薄束核TRPV 1-nNOS的表达参与了电针刺激通过体感传入-延髓通路的信号转导。
The purpose of this study was to determine the effects of electroacupuncture (EA) ST36 on food intake and body weight in Obese Prone (OP) rats compared to obese resistant (OR) strain on a high fat diet. The influences of EA on mRNA levels of pro-opiomelanocortin (POMC), transient receptor potential vanilloid type-1 (TRPV1), and neuronal nitric oxide synthase (nNOS) were also examined in the medulla regions and ST36 skin tissue. Advanced EA ST36 was conducted in two sessions of 20 min separated by an 80 min interval for 7 days. Food intake and body weight were recorded in conscious rats every day. Real time PCR was conducted in the micropunches of the medulla regions and skin tissues at the end of the treatment. Food intake and body weight were significantly reduced by advanced EA ST36 in OP rats, but slightly decreased in OR strain and sham-EA rats. Advanced EA ST36 produced a marked increase in POMC mRNA level in the nucleus tractus solitarius (NTS) and hypoglossal nucleus (HN) regions. TRPV1 and nNOS mRNAs were simultaneously increased in the NTS/gracile nucleus regions and in the ST36 skin regions by the EA treatment in OP rats. We conclude that advanced EA ST36 produces an up-regulation of anorexigenic factor POMC production in the NTS/HN, which inhibits food intake and reduces body weight. EA-induced expression of TRPV1-nNOS in the ST36 and the NTS/gracile nucleus is involved in the signal transduction of EA stimuli via somatosensory afferents-medulla pathways.
登录
查看更多内容
影响因子：
2.5
作者：
JOSEPH, SA;PILCHER, WH;BENNETTCLARKE, C
通讯作者：
BENNETTCLARKE, C
影响因子：
1.4
作者：
Koike, S;Uno, T;Hisa, Y
通讯作者：
Hisa, Y
影响因子：
5.3
作者：
MAMELI, O;TOLU, E
通讯作者：
TOLU, E
影响因子：
3.3
作者：
Miyamae, T;Goshima, Y;Misu, Y
通讯作者：
Misu, Y
影响因子：
3.8
作者：
Morrill, AC;Chinn, CD
通讯作者：
Chinn, CD