Gold-Oligonucleotide Nanoconstructs Engineered to Detect Conserved Enteroviral Nucleic Acid Sequences.
Gold-Oligonucleotide Nanoconstructs Engineered to Detect Conserved Enteroviral Nucleic Acid Sequences.
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DOI：
10.3390/bios11070238
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发表时间：
2021-07-14
期刊：
影响因子：
--
通讯作者：
Aylott JW
中科院分区：
文献类型：
--
作者：
Chauhan VM;Elsutohy MM;McClure CP;Irving WL;Roddis N;Aylott JW
肠道病毒是普遍存在的哺乳动物病原体，可产生轻度至危及生命的疾病。我们开发了一种多模式，快速，准确和经济的即时护理生物传感器，可以检测96%的所有已知肠道病毒中保守的核酸序列。该生物传感器利用金纳米颗粒和寡核苷酸的物理化学性质，提供对通过计算机筛选鉴定的独家肠道病毒核酸序列（23个碱基）的比色、光谱和基于侧流的鉴定。设计寡核苷酸以证明对靶肠道病毒核酸的特异性互补性，以产生聚集的金-寡核苷酸纳米构建体。保守的靶肠道病毒核酸序列（≥1 × 10−7 M，≥1.4 × 10−14 g/mL）启动金-寡核苷酸纳米构建体解聚和信号转导机制，产生比色和光谱蓝移（544 nm（紫色）> 524 nm（红色））。此外，利用金-寡核苷酸纳米构建体的侧向流测定不受污染的人类基因组DNA的影响，证明了保守的靶肠道病毒核酸序列的快速检测（<60 s），并且可以用定制的软件和硬件电子接口进行解释。我们预计，我们的方法将在计算机上筛选核酸数据库转化为有形的肠道病毒桌面检测器，它可以很容易地翻译为相关的生物体。这将为疾病的临床评估铺平道路，并补充现有的克服抗生素耐药性的战略。
Enteroviruses are ubiquitous mammalian pathogens that can produce mild to life-threatening disease. We developed a multimodal, rapid, accurate and economical point-of-care biosensor that can detect nucleic acid sequences conserved amongst 96% of all known enteroviruses. The biosensor harnesses the physicochemical properties of gold nanoparticles and oligonucleotides to provide colourimetric, spectroscopic and lateral flow-based identification of an exclusive enteroviral nucleic acid sequence (23 bases), which was identified through in silico screening. Oligonucleotides were designed to demonstrate specific complementarity towards the target enteroviral nucleic acid to produce aggregated gold–oligonucleotide nanoconstructs. The conserved target enteroviral nucleic acid sequence (≥1 × 10−7 M, ≥1.4 × 10−14 g/mL) initiates gold–oligonucleotide nanoconstruct disaggregation and a signal transduction mechanism, producing a colourimetric and spectroscopic blueshift (544 nm (purple) > 524 nm (red)). Furthermore, lateral-flow assays that utilise gold–oligonucleotide nanoconstructs were unaffected by contaminating human genomic DNA, demonstrated rapid detection of conserved target enteroviral nucleic acid sequence (<60 s), and could be interpreted with a bespoke software and hardware electronic interface. We anticipate that our methodology will translate in silico screening of nucleic acid databases to a tangible enteroviral desktop detector, which could be readily translated to related organisms. This will pave the way forward in the clinical evaluation of disease and complement existing strategies to overcome antimicrobial resistance.
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影响因子：
3.1
作者：
Huang, Yukun;Chen, Xiujuan;Wang, Zhouping
通讯作者：
Wang, Zhouping
影响因子：
12.4
作者：
Draz MS;Shafiee H
通讯作者：
Shafiee H
影响因子：
3.8
作者：
Hang VT;Nguyet NM;Trung DT;Tricou V;Yoksan S;Dung NM;Van Ngoc T;Hien TT;Farrar J;Wills B;Simmons CP
通讯作者：
Simmons CP
DOI：
10.3390/ph9040078
发表时间：
2016-12-16
期刊：
Pharmaceuticals (Basel, Switzerland)
影响因子：
--
作者：
González VM;Martín ME;Fernández G;García-Sacristán A
通讯作者：
García-Sacristán A
影响因子：
15
作者：
Hansen, JA;Wang, J;Collins, G
通讯作者：
Collins, G