PriFi: using a multiple alignment of related sequences to find primers for amplification of homologs.
PriFi: using a multiple alignment of related sequences to find primers for amplification of homologs.
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PRIFI:使用相关序列的多重比对来找到用于放大同源物的引物。
DOI:
10.1093/nar/gki425
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发表时间:
2005-07-01
影响因子:
14.9
通讯作者:
Stougaard, J
中科院分区:
文献类型:
--
作者:
Fredslund, J;Schauser, L;Madsen, LH;Sandal, N;Stougaard, J
Using a comparative approach, the web program PriFi () designs pairs of primers useful for PCR amplification of genomic DNA in species where prior sequence information is not available. The program works with an alignment of DNA sequences from phylogenetically related species and outputs a list of possibly degenerate primer pairs fulfilling a number of criteria, such that the primers have a maximal probability of amplifying orthologous sequences in other phylogenetically related species. Operating on a genome-wide scale, PriFi automates the first steps of a procedure for developing general markers serving as common anchor loci across species. To accommodate users with special preferences, configuration settings and criteria can be customized.
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影响因子:
30.8
作者:
Lyons, LA;Laughlin, TF;OBrien, SJ
通讯作者:
OBrien, SJ
DOI:
10.1073/pnas.0402251101
发表时间:
2004-10-26
影响因子:
11.1
作者:
Choi, HK;Mun, JH;Cook, DR
通讯作者:
Cook, DR
影响因子:
5.8
作者:
van Baren, MJ;Heutink, P
通讯作者:
Heutink, P
影响因子:
5.8
作者:
Pertea, G;Huang, XQ;Quackenbush, J
通讯作者:
Quackenbush, J
影响因子:
14.9
作者:
Haas, S;Vingron, M;Wiemann, S
通讯作者:
Wiemann, S