The effect of a nanofiber-hydrogel composite on neural tissue repair and regeneration in the contused spinal cord.
The effect of a nanofiber-hydrogel composite on neural tissue repair and regeneration in the contused spinal cord.
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DOI：
10.1016/j.biomaterials.2020.119978
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发表时间：
2020-07
期刊：
影响因子：
14
通讯作者：
Mao HQ
中科院分区：
文献类型：
--
作者：
Li X;Zhang C;Haggerty AE;Yan J;Lan M;Seu M;Yang M;Marlow MM;Maldonado-Lasunción I;Cho B;Zhou Z;Chen L;Martin R;Nitobe Y;Yamane K;You H;Reddy S;Quan DP;Oudega M;Mao HQ
脊髓损伤会导致神经组织的长期丢失，因为损伤部位的内源性神经组织修复和再生是有限的。我们设计了一种具有界面粘结的可注射纳米纤维-水凝胶复合材料(NHC)，以提供机械强度和孔隙度，并在成年大鼠脊髓挫伤模型中检测了其对修复和神经组织再生的影响。在NHC治疗28天后，挫伤脊髓节段的宽度是对照组的2倍。使用NHC治疗后，损伤组织的M2/M1巨噬细胞比率是对照组的2倍，血管密度是对照组的5倍，未成熟神经元的存在是对照组的2.6倍，轴突密度是对照组的2.4倍，神经胶质瘢痕的存在与对照组相似。而挫伤段备用神经组织体积和后肢功能组间差异无统计学意义。我们的发现表明，NHC为挫伤脊髓提供了机械支持，并在没有任何外源性因素或细胞的情况下，支持损伤组织中促再生巨噬细胞极化、血管生成、轴突生长和神经再生。这些结果促使进一步优化NHC和给药方案，以充分发挥NHC用于治疗脊髓损伤的独特特性的潜力。
An injury to the spinal cord causes long-lasting loss of nervous tissue because endogenous nervous tissue repair and regeneration at the site of injury is limited. We engineered an injectable nanofiber-hydrogel composite (NHC) with interfacial bonding to provide mechanical strength and porosity and examined its effect on repair and neural tissue regeneration in an adult rat model of spinal cord contusion. At 28 days after treatment with NHC, the width of the contused spinal cord segment was 2-fold larger than in controls. With NHC treatment, tissue in the injury had a 2-fold higher M2/M1 macrophage ratio, 5-fold higher blood vessel density, 2.6-fold higher immature neuron presence, 2.4-fold higher axon density, and a similar glial scar presence compared with controls. However, the spared nervous tissue volume in the contused segment and hind limb function was similar between groups. Our findings indicated that NHC provided mechanical support to the contused spinal cord and supported pro-regenerative macrophage polarization, angiogenesis, axon growth, and neurogenesis in the injured tissue without any exogenous factors or cells. These results motivate further optimization NHC and delivery protocol to fully translate the potential of the unique properties of NHC for treating spinal cord injury.
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