Unique nucleolar dominance patterns in distant hybrid lineage derived from Megalobrama Amblycephala × Culter Alburnus.
Unique nucleolar dominance patterns in distant hybrid lineage derived from Megalobrama Amblycephala × Culter Alburnus.
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来自团头鲂 x Culter Alburnus 的远缘杂交谱系中独特的核仁优势模式
DOI：
10.1186/s12863-016-0457-3
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发表时间：
2016-12-05
期刊：
影响因子：
2.9
通讯作者：
Liu S
中科院分区：
文献类型：
--
作者：
Xiao J;Hu F;Luo K;Li W;Liu S
核仁显性是一种表观遗传现象，发生在种间杂种中，并且涉及由于另一个祖先的rRNA基因的沉默而从一个祖先遗传的45 S rRNA基因的表达。本文研究了团头鲂（Megalobrama amblycephala）×翘嘴卡尔特（TC，卡尔特alburnus）杂种F1和F2代的遗传和45 S rRNA基因表达的变化。通过克隆、RT-PCR和测序方法对45 SrDNA位点进行分析。结果表明，杂种F_1和F_2的核仁优势模式不同。在BSB × TC的F1中，所有被测个体均遗传并表达了BSB和TC的45 SrRNA基因，表明在F1中未建立核仁优势。而BSB × TC的F_2则表现出5种类型。模式1仅遗传并表达BSB的45 S rRNA基因。模式2继承了BSB和TC的45 SrRNA基因，但仅表达BSB的45 SrRNA。模式3继承并表达来自BSB和TC的45 S rRNA基因。模式4遗传了BSB和TC的45 SrRNA基因，但只表达TC的45 SrRNA基因。模式5仅遗传并表达TC的45 S rRNA基因。在BSB × TC的属间杂种中，核仁优势表现出独特的模式。在F_1中不成立，在F_2中是随机的。这项研究为脊椎动物遗传杂种中的核仁优势现象提供了新的见解。本文的在线版本（doi：10.1186/s12863-016-0457-3）包含补充材料，可供授权用户使用。
Nucleolar dominance is an epigenetic phenomenon that occurs in interspecific hybrids and involves the expression of 45S rRNA genes inherited from one progenitor due to the silencing of the other progenitor’s rRNA genes. In this paper, changes in the genetics and expression of 45S rRNA genes in F1 and F2 hybrid progeny of blunt snout bream (BSB, Megalobrama amblycephala) × topmouth culter (TC, Culter alburnus) are investigated. The 45S rDNA loci were analyzed by cloning, RT-PCR and sequencing methods. The results show that nucleolar dominance patterns differ in the F1 and F2 hybrids. In the F1 hybrids of BSB × TC, all the tested individuals inherited and expressed the 45S rRNA genes of both BSB and TC, indicating that nucleolar dominance is not established in the F1 hybrids. However, in the F2 hybrids of BSB × TC, five patterns are observed. Pattern 1 inherits and expresses only the 45S rRNA gene of BSB. Pattern 2 inherits the 45S rRNA gene from both BSB and TC, but only expresses the 45S rRNA of BSB. Pattern 3 inherits and expresses the 45S rRNA gene from both BSB and TC. Pattern 4 inherits the 45S rRNA gene from both BSB and TC, but only expresses the 45S rRNA gene of TC. Pattern 5 inherits and expresses only the 45S rRNA gene of TC. Nucleolar dominance shows distinctive patterns in intergeneric hybrids of BSB × TC. It is not established in F1 hybrids and is random in F2 hybrids. This study provides new insights into the phenomenon of nucleolar dominance in genetic hybrids in vertebrates. The online version of this article (doi:10.1186/s12863-016-0457-3) contains supplementary material, which is available to authorized users.
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