Extracellular Vesicle-Associated mir-21 and mir-144 Are Markedly Elevated in Serum of Patients With Hepatocellular Carcinoma.
Extracellular Vesicle-Associated mir-21 and mir-144 Are Markedly Elevated in Serum of Patients With Hepatocellular Carcinoma.
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肝细胞癌患者血清细胞外囊泡相关mir-21和mir-144显著升高
DOI：
10.3389/fphys.2018.00930
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发表时间：
2018
影响因子：
4
通讯作者：
Shao S
中科院分区：
文献类型：
--
作者：
Pu C;Huang H;Wang Z;Zou W;Lv Y;Zhou Z;Zhang Q;Qiao L;Wu F;Shao S
Aim: The aim of this study was to observe the possible change of microRNAs (miRNAs) in serum extracellular vesicles (EVs) from hepatocellular carcinoma (HCC) patients. Methods: The serum EVs were purified from 17 healthy donors, 16 chronic hepatitis B (CHB) patients and 24 HCC patients. The sequenced microRNAs in the purified EVs were analyzed to obtain highly differentially expressed genes (DEGs). Finally, the expression pattern of DEGs was validated using qRT-PCR. Results: We found that the expression of hsa-miR-21-5p and hsa-miR-144-3p were significantly higher in EVs and liver cancer tissues compared with serum and the distal liver tissues in HCC patients. The ratio of hsa-miR-144-3p/hsa-miR-21-5p was significantly decreased in the patients with CHB but significantly increased in patients with HCC developed from CHB (P < 0.05). Hsa-144-3p/hsa-miR-21-5p exhibited greater performance than alpha-fetoprotein (AUC 0.780, 95% CI 0.601–0.960, versus AUC 0.626, 95% CI 0.410–0.843) in ROC curve analysis. Conclusion: Extracellular vesicle-associated hsa-miR-21-5p and hsa-miR-144-3p are markedly elevated in serum of patients with HCC. The potential role of these microRNAs in the pathogenesis of HCC is worth of further study.
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影响因子：
4
作者：
Masoudi, Mohammad Sadegh;Mehrabian, Emadodin;Mirzaei, Hamed
通讯作者：
Mirzaei, Hamed
影响因子：
21.3
作者：
Skog, Johan;Wuerdinger, Tom;van Rijn, Sjoerd;Meijer, Dimphna H.;Gainche, Laura;Sena-Esteves, Miguel;Curry, William T., Jr.;Carter, Bob S.;Krichevsky, Anna M.;Breakefield, Xandra O.
通讯作者：
Breakefield, Xandra O.
影响因子：
9.5
作者：
Li, Jing;Rohailla, Sagar;Redington, Andrew N.
通讯作者：
Redington, Andrew N.
影响因子：
3.4
作者：
Asea, Alexzander;Jean-Pierre, Claudel;Witkin, Steven S.
通讯作者：
Witkin, Steven S.
Vps4A 通过调节人肝癌细胞中外泌体 MicroRNA 的分泌和摄取发挥肿瘤抑制作用
DOI：
10.1002/hep.27660
发表时间：
2015-04
期刊：
Hepatology (Baltimore, Md.)
影响因子：
--
作者：
Wei JX;Lv LH;Wan YL;Cao Y;Li GL;Lin HM;Zhou R;Shang CZ;Cao J;He H;Han QF;Liu PQ;Zhou G;Min J
通讯作者：
Min J