Biotransformation of Panax ginseng extract by rat intestinal microflora: identification and quantification of metabolites using liquid chromatography-tandem mass spectrometry.
Biotransformation of Panax ginseng extract by rat intestinal microflora: identification and quantification of metabolites using liquid chromatography-tandem mass spectrometry.
复制标题
大鼠肠道微生物群对人参提取物的生物转化：使用液相色谱-串联质谱法鉴定和定量代谢物x
DOI：
10.1016/j.jgr.2016.11.002
复制
发表时间：
2017-10
影响因子：
6.3
通讯作者：
Quan LH
中科院分区：
文献类型：
--
作者：
Dong WW;Zhao J;Zhong FL;Zhu WJ;Jiang J;Wu S;Yang DC;Li D;Quan LH
一般来说，口服人参后，肠道微生物在其降解代谢过程中起着至关重要的作用。利用微生物区系研究人参的代谢，对于更好地了解其生物学效应具有重要意义。研究了人参提取液在37℃、24 h条件下大鼠肠道菌群对人参提取液的生物转化作用，并用LC-MS/MS法同时测定了大鼠肠道菌群对人参皂苷的代谢产物和代谢谱。在转化的人参样品中，检测到相当数量的脱糖代谢物化合物K和Rh1。此外，在24小时还检测到微量的脱糖代谢产物(人参皂苷Rg2、F1、F2、Rg3和原人参三醇型人参皂苷)和未转化的人参皂苷Re、Rg1和Rd，结果表明，主要代谢产物为化合物K和Rh1，且原人参二醇型人参皂苷比原人参三醇型人参皂苷更容易代谢。这是首次利用LC-MS/MS对人参提取物在大鼠肠道菌群中的代谢和代谢谱进行鉴定和定量的报道，为研究人参的代谢和活性代谢物提供了新的思路。
In general, after Panax ginseng is administered orally, intestinal microbes play a crucial role in its degradation and metabolization process. Studies on the metabolism of P. ginseng by microflora are important for obtaining a better understanding of their biological effects. In vitro biotransformation of P. ginseng extract by rat intestinal microflora was investigated at 37°C for 24 h, and the simultaneous determination of the metabolites and metabolic profile of P. ginseng saponins by rat intestinal microflora was achieved using LC–MS/MS. A total of seven ginsenosides were detected in the P. ginseng extract, including ginsenosides Rg1, Re, Rf, Rb1, Rc, Rb2, and Rd. In the transformed P. ginseng samples, considerable amounts of deglycosylated metabolite compound K and Rh1 were detected. In addition, minimal amounts of deglycosylated metabolites (ginsenosides Rg2, F1, F2, Rg3, and protopanaxatriol-type ginsenosides) and untransformed ginsenosides Re, Rg1, and Rd were detected at 24 h. The results indicated that the primary metabolites are compound K and Rh1, and the protopanaxadiol-type ginsenosides were more easily metabolized than protopanaxatriol-type ginsenosides. This is the first report of the identification and quantification of the metabolism and metabolic profile of P. ginseng extract in rat intestinal microflora using LC–MS/MS. The current study provided new insights for studying the metabolism and active metabolites of P. ginseng.
登录
查看更多内容
人类肠道细菌通过绞股蓝皂苷途径生物转化人参皂苷 Rb1
影响因子：
4.9
作者：
Shen, Hong;Leung, Weng-Im;Yan, Ru
通讯作者：
Yan, Ru
影响因子：
4.4
作者：
Shin, HY;Park, SY;Kim, DH
通讯作者：
Kim, DH
影响因子：
2
作者：
Shin, HY;Lee, JH;Kim, DH
通讯作者：
Kim, DH
影响因子：
6.3
作者：
通讯作者：
--
影响因子：
6.3
作者：
Kim, Kyung-Ah;Yoo, Hye Hyun;Gu, Wan;Yu, Dae-Hyung;Jin, Ming Ji;Choi, Hae-Lim;Yuan, Kathy;Guerin-Deremaux, Laetitia;Kim, Dong-Hyun
通讯作者：
Kim, Dong-Hyun