Stereospecific anticancer effects of ginsenoside Rg3 epimers isolated from heat-processed American ginseng on human gastric cancer cell.
Stereospecific anticancer effects of ginsenoside Rg3 epimers isolated from heat-processed American ginseng on human gastric cancer cell.
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DOI：
10.1016/j.jgr.2013.11.007
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发表时间：
2014-01
影响因子：
6.3
通讯作者：
中科院分区：
文献类型：
--
作者：
已经进行了研究，开发通过高温或高压加工转化人参皂苷（人参的主要活性成分）来提高人参药效的方法。本研究旨在探讨热处理西洋参 (HAG) 对人胃癌 AGS 细胞的抗癌作用，重点评估细胞凋亡作为其抗癌作用中重要机制因素的作用。 HAG显着减少癌细胞增殖，人参皂苷Rb1和Re的含量显着降低，而新检测到弱极性人参皂苷[20(S,R)-Rg3、Rk1和Rg5]的峰。基于HAG的活性引导分级分离，人参皂苷20(S)-Rg3在诱导人胃癌AGS细胞凋亡中发挥关键作用，其主要由人参皂苷Rb1产生。人参皂苷 20(S)-Rg3 通过激活 caspase-3、caspase-8 和 caspase-9 以及调节 Bcl-2 和 Bax 表达来诱导细胞凋亡。综上所述，这些研究结果表明，热处理可增强西洋参在 AGS 细胞中的抗肿瘤活性，而热处理产生的活性成分人参皂苷 20(S)-Rg3 会诱导 caspase-3、caspase-8 和 caspase-9 的激活，从而导致细胞凋亡。
Research has been conducted with regard to the development of methods for improving the pharmaceutical effect of ginseng by conversion of ginsenosides, which are the major active components of ginseng, via high temperature or high-pressure processing. The present study sought to investigate the anticancer effect of heat-processed American ginseng (HAG) in human gastric cancer AGS cells with a focus on assessing the role of apoptosis as an important mechanistic element in its anticancer actions. HAG significantly reduced the cancer cell proliferation, and the contents of ginsenosides Rb1 and Re were markedly decreased, whereas the peaks of less-polar ginsenosides [20(S,R)-Rg3, Rk1, and Rg5] were newly detected. Based on the activity-guided fractionation of HAG, ginsenoside 20(S)-Rg3 played a key role in inducing apoptosis in human gastric cancer AGS cells, and it was generated mainly from ginsenoside Rb1. Ginsenoside 20(S)-Rg3 induced apoptosis through activation of caspase-3, caspase-8, and caspase-9, as well as regulation of Bcl-2 and Bax expression. Taken together, these findings suggest that heat-processing serves as an increase in the antitumor activity of American ginseng in AGS cells, and ginsenoside 20(S)-Rg3, the active component produced by heat-processing, induces the activation of caspase-3, caspase-8, and caspase-9, which contributes to the apoptotic cell death.
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影响因子：
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