A prebiotic fiber increases the formation and subsequent absorption of compound K following oral administration of ginseng in rats.
A prebiotic fiber increases the formation and subsequent absorption of compound K following oral administration of ginseng in rats.
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DOI：
10.1016/j.jgr.2014.11.002
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发表时间：
2015-04
影响因子：
6.3
通讯作者：
Kim, Dong-Hyun
中科院分区：
文献类型：
--
作者：
Kim, Kyung-Ah;Yoo, Hye Hyun;Gu, Wan;Yu, Dae-Hyung;Jin, Ming Ji;Choi, Hae-Lim;Yuan, Kathy;Guerin-Deremaux, Laetitia;Kim, Dong-Hyun
肠道菌群在人参皂苷转化为化合物K （CK）的过程中起着至关重要的作用，并可能影响人参的药理作用。NUTRIOSE等益生元通过调节肠道微生物代谢活性，促进CK的形成和吸收。本研究研究了一种益生元纤维（NUTRIOSE）对人参皂苷生物活性代谢物人参皂苷CK的药动学影响及其作用机制。雄性Sprague-Dawley大鼠分别饲喂对照或含NUTRIOSE饲粮（对照饲粮+ NUTRIOSE） 2周，然后口服人参提取物或参物。采集血样，采用液相色谱-串联质谱法研究CK的药代动力学。用粪便标本或细菌培养物测定粪便代谢人参皂苷Rb1到CK的活性。人参提取物分别饲喂含NUTRIOSE 2.5%、5%和10%饲粮的大鼠，CK的最大血浆浓度（Cmax）和血浆浓度-时间曲线下面积显著增加，且呈NUTRIOSE含量依赖关系。营养糖的摄入增加了大鼠肠内容物中糖苷酶活性和CK的形成。NUTRIOSE对体外培养的肠道菌群ck形成活性有显著的诱导作用。综上所述，饲粮中添加NUTRIOSE等益生元可促进人参皂苷代谢转化为CK，并促进CK在胃肠道的吸收，从而增强人参的药理作用。
Gut microflora play a crucial role in the biotransformation of ginsenosides to compound K (CK), which may affect the pharmacological effects of ginseng. Prebiotics, such as NUTRIOSE, could enhance the formation and consequent absorption of CK through the modulation of gut microbial metabolic activities. In this study, the effect of a prebiotic fiber (NUTRIOSE) on the pharmacokinetics of ginsenoside CK, a bioactive metabolite of ginsenosides, and its mechanism of action were investigated. Male Sprague–Dawley rats were given control or NUTRIOSE-containing diets (control diet + NUTRIOSE) for 2 wk, and ginseng extract or vehicle was then orally administered. Blood samples were collected to investigate the pharmacokinetics of CK using liquid chromatography–tandem mass spectrometry. Fecal activities that metabolize ginsenoside Rb1 to CK were assayed with fecal specimens or bacteria cultures. When ginseng extract was orally administered to rats fed with 2.5%, 5%, or 10% NUTRIOSE containing diets, the maximum plasma concentration (Cmax) and area under the plasma concentration–time curve values of CK significantly increased in a NUTRIOSE content-dependent manner. NUTRIOSE intake increased glycosidase activity and CK formation in rat intestinal contents. The CK-forming activities of intestinal microbiota cultured in vitro were significantly induced by NUTRIOSE. These results show that prebiotic diets, such as NUTRIOSE, may promote the metabolic conversion of ginsenosides to CK and the subsequent absorption of CK in the gastrointestinal tract and may potentiate the pharmacological effects of ginseng.
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影响因子：
6.3
作者：
Kim JS;Kim Y;Han SH;Jeon JY;Hwang M;Im YJ;Kim JH;Lee SY;Chae SW;Kim MG
通讯作者：
Kim MG
DOI：
10.1007/bf03188922
发表时间：
1994-07-01
影响因子：
1.9
作者：
MIKOV, M
通讯作者：
MIKOV, M
DOI：
10.1111/nyas.12303
发表时间：
2013-01-01
期刊：
ANNALS REPORTS
影响因子：
--
作者：
Petschow, Bryon;Dore, Joel;Sanders, Mary Ellen
通讯作者：
Sanders, Mary Ellen
影响因子：
2
作者：
Bae, EA;Park, SY;Kim, DH
通讯作者：
Kim, DH
DOI：
10.1006/bbrc.1998.8690
发表时间：
1998-05-29
影响因子：
3.1
作者：
Wakabayashi, C;Murakami, K;Saiki, I
通讯作者：
Saiki, I