The RdDM pathway is required for basal heat tolerance in Arabidopsis.
The RdDM pathway is required for basal heat tolerance in Arabidopsis.
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DOI：
10.1093/mp/sst023
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发表时间：
2013-03
期刊：
影响因子：
27.5
通讯作者：
Jonak C
中科院分区：
文献类型：
--
作者：
Popova OV;Dinh HQ;Aufsatz W;Jonak C
这项工作表明，成功的热响应取决于表观遗传途径的完整性，并提供证据表明，热依赖性基因表达受到位置接近的转座子序列和通读转录的影响。热胁迫影响拟南芥表观遗传基因沉默。为了测试热应激反应中表观遗传调控的机制参与，我们分析了DNA甲基化、组蛋白修饰、染色质重塑或基于siRNA的沉默途径缺陷突变体的耐热性。NRPD2（RNA聚合酶IV和V的常见第二大亚基）和Rpd3型组蛋白去乙酰化酶HDA6缺陷的植物对热暴露过敏。基因芯片分析表明，NRPD2和HDA6在温度胁迫下的转录重编程中具有独立的作用。从热恢复的nrpd2突变体中蛋白质编码基因的错误表达与相邻转座子残余物的表观遗传调控缺陷相关，所述转座子残余物涉及热应激诱导的通读转录失控。我们提供的证据表明，温度胁迫的转录反应，至少部分依赖于RNA依赖的DNA甲基化途径的完整性。
This work shows that a successful heat response depends on the integrity of epigenetic pathways and provides evidence that heat-dependent gene expression is influenced by closely located transposon sequences and read-through transcription. Heat stress affects epigenetic gene silencing in Arabidopsis. To test for a mechanistic involvement of epigenetic regulation in heat-stress responses, we analyzed the heat tolerance of mutants defective in DNA methylation, histone modifications, chromatin-remodeling, or siRNA-based silencing pathways. Plants deficient in NRPD2, the common second-largest subunit of RNA polymerases IV and V, and in the Rpd3-type histone deacetylase HDA6 were hypersensitive to heat exposure. Microarray analysis demonstrated that NRPD2 and HDA6 have independent roles in transcriptional reprogramming in response to temperature stress. The misexpression of protein-coding genes in nrpd2 mutants recovering from heat correlated with defective epigenetic regulation of adjacent transposon remnants which involved the loss of control of heat-stress-induced read-through transcription. We provide evidence that the transcriptional response to temperature stress, at least partially, relies on the integrity of the RNA-dependent DNA methylation pathway.
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影响因子：
10.5
作者：
Bartee, L;Malagnac, F;Bender, J
通讯作者：
Bender, J
影响因子：
5.3
作者：
Cramer GR;Urano K;Delrot S;Pezzotti M;Shinozaki K
通讯作者：
Shinozaki K
影响因子：
7.4
作者：
Haberer, G;Young, S;Messing, J
通讯作者：
Messing, J
DOI：
10.1016/j.bbagrm.2011.03.007
发表时间：
2011-08
影响因子：
4.7
作者：
Hauser, Marie-Theres;Aufsatz, Werner;Jonak, Claudia;Luschnig, Christian
通讯作者：
Luschnig, Christian
影响因子：
30.8
作者：
Dunoyer, Patrice;Himber, Christophe;Voinnet, Olivier
通讯作者：
Voinnet, Olivier