A translation initiation element specific to mRNAs with very short 5'UTR that also regulates transcription.
A translation initiation element specific to mRNAs with very short 5'UTR that also regulates transcription.
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DOI：
10.1371/journal.pone.0003094
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发表时间：
2008-08-28
期刊：
影响因子：
3.7
通讯作者：
Dikstein, Rivka
中科院分区：
文献类型：
--
作者：
Elfakess, Rofa;Dikstein, Rivka
转录是由顺式调控元件控制的，如果顺式调控元件位于转录起始位点（TSS）下游的5'UTR中，也可能影响翻译。然而，目前很少有证据表明这种复合监管要素。我们通过计算分析确定了位于TSS下游+30位置的丰富元件，该元件控制转录和翻译。该元件具有一个不变的ATG序列，在携带它的64%的基因中作为翻译起始密码子。在这些基因中，起始AUG之前有一个极短的5'UTR。我们发现，在体外和体内的翻译完全由该基序的AUG启动，并且AUG侧翼序列创建了一个强大的翻译启动上下文。该基序与著名的Kozak基序的区别在于其独特的能力，可以指导具有非常短的5'UTR的mrna高效准确的翻译起始。因此，我们将其命名为TISU，即短5'UTR的翻译启动器。有趣的是，这个翻译起始元件也是阴阳1号的重要转录调控元件。我们对一个共同的转录和翻译元件的描述指出了哺乳动物转录和翻译起始之间的联系。
Transcription is controlled by cis regulatory elements, which if localized downstream to the transcriptional start site (TSS), in the 5′UTR, could influence translation as well. However presently there is little evidence for such composite regulatory elements. We have identified by computational analysis an abundant element located downstream to the TSS up to position +30, which controls both transcription and translation. This element has an invariable ATG sequence, which serves as the translation initiation codon in 64% of the genes bearing it. In these genes the initiating AUG is preceded by an extremely short 5′UTR. We show that translation in vitro and in vivo is initiated exclusively from the AUG of this motif, and that the AUG flanking sequences create a strong translation initiation context. This motif is distinguished from the well-known Kozak in its unique ability to direct efficient and accurate translation initiation from mRNAs with a very short 5′UTR. We therefore named it TISU for Translation Initiator of Short 5′UTR. Interestingly, this translation initiation element is also an essential transcription regulatory element of Yin Yang 1. Our characterization of a common transcription and translation element points to a link between mammalian transcription and translation initiation.
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影响因子：
4.8
作者：
Amir-Zilberstein, Liat;Dikstein, Rivka
通讯作者：
Dikstein, Rivka
影响因子：
14.9
作者：
LEE, JS;SEE, RH;SHI, Y
通讯作者：
SHI, Y
影响因子：
3.1
作者：
LEE, YM;LEE, SC
通讯作者：
LEE, SC
影响因子：
12.3
作者：
Schug J;Schuller WP;Kappen C;Salbaum JM;Bucan M;Stoeckert CJ Jr
通讯作者：
Stoeckert CJ Jr
DOI：
10.1073/pnas.88.21.9804
发表时间：
1991-11-01
影响因子：
11.1
作者：
PARK, K;ATCHISON, ML
通讯作者：
ATCHISON, ML