The homotetrameric kinesin-5 KLP61F preferentially crosslinks microtubules into antiparallel orientations.
The homotetrameric kinesin-5 KLP61F preferentially crosslinks microtubules into antiparallel orientations.
复制标题
同型动力蛋白-5 klp61f优先将微管交联成反平行方向。
DOI：
10.1016/j.cub.2008.10.026
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发表时间：
2008-12-09
期刊：
影响因子：
9.2
通讯作者：
Peterman, Erwin J. G.
中科院分区：
文献类型：
--
作者：
van den Wildenberg, Siet M. J. L.;Tao, Li;Kapitein, Lukas C.;Schmidt, Christoph F.;Scholey, Jonathan M.;Peterman, Erwin J. G.
有丝分裂过程中遗传物质的分离由有丝分裂纺锤体协调，其作用机制取决于其组成的微管（MTs）的极性模式。同四聚体有丝分裂运动蛋白-5马达能够交联和滑动相邻的纺锤体MT，但尚不清楚它们或其他马达是否有助于这些MT极性模式的建立。在这里，我们研究了果蝇胚胎运动蛋白5 KLP61F，它被认为可以交联平行和反平行的mt，是否对mt的平行或反平行取向表现出偏好。在运动实验中，KLP61F被观察到交联和滑动相邻的mt，正如预测的那样。值得注意的是，相对于平行取向，KLP61F对反平行取向交联mt的偏好高3倍。这种极性偏好在ADP或ATP加AMPPNP存在时观察到，但在单独的AMPPNP中没有观察到，后者会诱导瞬时严格结合。此外，含有c端尾部结构域的纯化无运动四聚体显示出反平行取向偏好，证实运动活性不是必需的。结果表明，在果蝇胚胎有丝分裂纺锤体的形态发生过程中，KLP61F的交联和滑动活动可以促进KLP61F在赤道的反平行极间（ip） mt内的逐渐积累，从而产生动力驱动极性通量和两极分离。
The segregation of the genetic material during mitosis is coordinated by the mitotic spindle, whose mechanism of action depends upon the polarity patterns of its constituent microtubules (MTs). Homotetrameric mitotic kinesin-5 motors are capable of crosslinking and sliding adjacent spindle MTs, but it is unknown if they, or other motors, contribute to the establishment of these MT polarity patterns. Here we explored if the Drosophila embryo kinesin-5, KLP61F, which is thought to crosslink both parallel and anti-parallel MTs, displays a preference for the parallel or anti-parallel orientation of MTs. In motility assays, KLP61F was observed to crosslink and slide adjacent MTs, as predicted. Remarkably, KLP61F displayed a three-fold higher preference for crosslinking MTs in the antiparallel, relative to the parallel orientation. This polarity preference was observed in the presence of ADP or in ATP plus AMPPNP, but not in AMPPNP alone, which induces instantaneous rigor binding. Also, a purified motorless tetramer containing the C-terminal tail domains displayed an antiparallel orientation preference, confirming that motor activity is not required. The results suggest that, during the morphogenesis of the Drosophila embryo mitotic spindle, the crosslinking and sliding activities of KLP61F could facilitate the gradual accumulation of KLP61F within antiparallel interpolar (ip) MTs at the equator, where the motor could then generate force to drive poleward flux and pole-pole separation.
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DOI：
10.1073/pnas.92.10.4289
发表时间：
1995-05-09
影响因子：
11.1
作者：
SAWIN, KE;MITCHISON, TJ
通讯作者：
MITCHISON, TJ
DOI：
10.1083/jcb.143.3.687
发表时间：
1998-11-02
期刊：
The Journal of cell biology
影响因子：
--
作者：
Straight AF;Sedat JW;Murray AW
通讯作者：
Murray AW
影响因子：
4.8
作者：
Hildebrandt, Emily R.;Gheber, Larisa;Hoyt, M. Andrew
通讯作者：
Hoyt, M. Andrew
影响因子：
64.8
作者：
Kashina, AS;Baskin, RJ;Scholey, JM
通讯作者：
Scholey, JM
DOI：
10.1083/jcb.105.2.875
发表时间：
1987-08
期刊：
The Journal of cell biology
影响因子：
--
作者：
Saxton WM;McIntosh JR
通讯作者：
McIntosh JR