Autofluorescence hyperspectral imaging of radiofrequency ablation lesions in porcine cardiac tissue.
Autofluorescence hyperspectral imaging of radiofrequency ablation lesions in porcine cardiac tissue.
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DOI：
10.1002/jbio.201600071
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发表时间：
2017-08
影响因子：
2.8
通讯作者：
Sarvazyan NA
中科院分区：
文献类型：
--
作者：
Gil DA;Swift LM;Asfour H;Muselimyan N;Mercader MA;Sarvazyan NA
房颤是最常见的心律失常，射频消融(RFA)是一种广泛使用的治疗方法。在这里，我们探索自体荧光高光谱成像(AHSI)作为一种方法，以显示RFA病变和高度胶原的左心房间隙。用紫外光(365 Nm)照射新鲜切除的猪左房心内膜表面的RFA损伤，获得可见范围(420~720 nm)的高光谱数据立方体。使用线性分解法从周围组织中勾勒出RFA病变，并将来自非混合成分图像的病变直径与大体病理进行定量比较。RFA在自发荧光发射谱中引起了两个一致的变化：在490 nm以下波长的减少(归因于内源性NADH的损失)和在490 nm以上的波长的增加(归因于散射增加)。这些光谱变化使得能够高分辨率地、原位地描绘RFA病变边界，而不需要额外的染色或外源标记。我们的结果证实了使用aHSI对临床相关部位的RFA病变进行可视化的可行性。如果集成到经皮可视导管中，aHSI将在RFA过程中实现广泛的光学外科指导，并可以通过减少心房颤动的复发来改善患者预后。
Radiofrequency ablation (RFA) is a widely used treatment for atrial fibrillation, the most common cardiac arrhythmia. Here, we explore autofluorescence hyperspectral imaging (aHSI) as a method to visualize RFA lesions and interlesional gaps in the highly collagenous left atrium. RFA lesions made on the endocardial surface of freshly excised porcine left atrial tissue were illuminated by UV light (365 nm), and hyperspectral datacubes were acquired over the visible range (420–720 nm). Linear unmixing was used to delineate RFA lesions from surrounding tissue, and lesion diameters derived from unmixed component images were quantitatively compared to gross pathology. RFA caused two consistent changes in the autofluorescence emission profile: a decrease at wavelengths below 490 nm (ascribed to a loss of endogenous NADH) and an increase at wavelengths above 490 nm (ascribed to increased scattering). These spectral changes enabled high resolution, in situ delineation of RFA lesion boundaries without the need for additional staining or exogenous markers. Our results confirm the feasibility of using aHSI to visualize RFA lesions at clinically relevant locations. If integrated into a percutaneous visualization catheter, aHSI would enable widefield optical surgical guidance during RFA procedures and could improve patient outcome by reducing atrial fibrillation recurrence.
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影响因子：
37.8
作者：
Chen, SA;Hsieh, MH;Chang, MS
通讯作者：
Chang, MS
DOI：
10.1111/j.1540-8167.2006.00594.x
发表时间：
2006-10-01
影响因子：
2.7
作者：
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通讯作者：
Schilling, Richard J.
影响因子：
3.5
作者：
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通讯作者：
Frangioni, John V.
影响因子：
2.8
作者：
Ahmad, Iftikhar;Gribble, Adam;Vitkin, Alex
通讯作者：
Vitkin, Alex
影响因子：
8.4
作者：
Cappato, Riccardo;Calkins, Hugh;Biganzoli, Elia
通讯作者：
Biganzoli, Elia