Reexamination of human T cell lymphotropic virus (HTLV-I/II) prevalence.
Reexamination of human T cell lymphotropic virus (HTLV-I/II) prevalence.
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人类 T 细胞嗜淋巴细胞病毒 (HTLV-I/II) 流行率的重新检查。
DOI：
10.1073/pnas.94.12.6403
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发表时间：
1997
影响因子：
11.1
通讯作者：
Legler,PM
中科院分区：
文献类型：
--
作者：
Zucker-Franklin,D;Pancake,BA;Marmor,M;Legler,PM
在美国，献血者正在接受血清学筛查，检查是否感染人类T细胞嗜淋巴病毒I和II (HTLV-I/II)，血清学检测这些病毒结构蛋白的抗体。由于蕈样真菌病（MF）患者通常没有这种抗体，即使他们的细胞含有HTLV- i Tax和/或pol前病毒序列，因此人们质疑，目前的检测手段是否也低估了健康献血者中HTLV感染的流行程度。为了检验这种可能性，对蕈样真菌病患者（MFR）的亲属标本进行了研究。此外，为了更快地收集数据，我们对一组前注射吸毒者（IDUs）进行了常规血清学检测，并对Tax、pol和gag前病毒序列进行了PCR/Southern blot分析，对Tax基因产物的抗体进行了Western blot分析。迄今为止，6/8 MFR和42/81 (51.8%)hiv阴性IDUs被证实HTLV阳性，而常规血清学未发现MFR，只有18/81 (22.2%)IDUs。在后者的测试对象中，HTLV-I的发病率也比预期高10倍。因此，在健康的献血者中，HTLV-I/II感染可能比目前认为的更为普遍。由于Tax是HTLV-I/II的转化序列，因此在输血和组织供体设施中可能需要检测Tax序列及其基因产物的抗体。
In the United States, blood donors are being screened for infection with human T cell lymphotropic viruses I and II (HTLV-I/II) by serologic means, which detect antibodies to the structural proteins of these viruses. Because patients with mycosis fungoides (MF) usually do not have such antibodies even though their cells harbor HTLV-I Tax and/or pol proviral sequences, it was questioned whether the prevalence of HTLV infection among healthy blood donors may also be underestimated by current means of testing. To examine this possibility, a study on specimens of relatives of mycosis fungoides patients (MFR) was begun. In addition, to collect data more expeditiously, a cohort of former injection drug users (IDUs) was tested by routine serologic methods, as well as by PCR/Southern blot analysis for Tax, pol, and gag proviral sequences and Western blot analysis for antibodies to the Tax gene product. To date, 6/8 MFRs and 42/81 (51.8%) of HIV-negative IDUs proved to be positive for HTLV, whereas routine serology identified none of the MFR and only 18/81 (22.2%) of the IDUs. Among the latter test subjects, the incidence of HTLV-I also proved to be 10 times higher than expected. Therefore, it is likely that among healthy blood donors infection with HTLV-I/II is more prevalent than is currently assumed. Since Tax is the transforming sequence of HTLV-I/II, testing for Tax sequences and antibodies to its gene product may be desirable in blood transfusion and tissue donor facilities.
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影响因子：
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作者：
Sunzeri,FJ;Lee,TH;Brownlee,RG;Busch,MP
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作者：
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影响因子：
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DOI：
10.1097/00042560-199612010-00003
发表时间：
1996
期刊：
Journal of acquired immune deficiency syndromes and human retrovirology : official publication of the International Retrovirology Association
影响因子：
--
作者：
Pancake,BA;Zucker-Franklin,D
通讯作者：
Zucker-Franklin,D