Arginine Vasopressin and Arginine Vasopressin Receptor 1b Involved in Electroacupuncture-Attenuated Hypothalamic-Pituitary-Adrenal Axis Hyperactivity in Hepatectomy Rats.
Arginine Vasopressin and Arginine Vasopressin Receptor 1b Involved in Electroacupuncture-Attenuated Hypothalamic-Pituitary-Adrenal Axis Hyperactivity in Hepatectomy Rats.
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精氨酸加压素和精氨酸加压素受体 1b 参与电针减弱肝切除大鼠下丘脑 - 垂体 - 肾上腺轴过度活跃
DOI：
10.1111/ner.12366
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发表时间：
2016-07
期刊：
影响因子：
--
通讯作者：
Tian Z
中科院分区：
文献类型：
--
作者：
Zhu J;Chen Z;Zhu L;Meng Z;Wu G;Tian Z
本研究旨在了解电针（EA）在维持肝切除大鼠神经内分泌系统内稳态中的作用，并观察电针后下丘脑中精氨酸加压素（AVP）信号通路的参与情况。
将大鼠随机分为四组，包括完整组、模型组、假电针组和电针组。肝切除术后在围手术期对足三里（ST36）和三阴交（SP6）穴位进行电针。通过放射免疫分析法检测血清促肾上腺皮质激素（ACTH）和皮质酮（CORT）水平。术后采用蛋白质印迹法检测AVP、精氨酸加压素受体1a（AVPR1a）、精氨酸加压素受体1b（AVPR1b）和糖皮质激素受体（GR）的表达。
与完整组相比，模型组血清中ACTH和CORT水平升高，而电针组与模型组相比，ACTH和CORT水平降低。此外，模型组垂体中AVP和AVPR1b蛋白水平升高，电针组则降低。进一步观察到，模型组中AVP和AVPR1a蛋白水平显著升高，而电针组中则显著降低。奈立膦坦阻断AVPR1b可降低ACTH和CORT的升高。[亮氨酸4，赖氨酸8]加压素可抑制电针纠正下丘脑 - 垂体 - 肾上腺（HPA）轴亢进的作用。
在ST36和SP6穴位应用电针可在围手术期通过AVP信号通路改善HPA轴的亢进。
The study aims to know the effect of electroacupuncture (EA) in maintenance of the homeostasis of the neuroendocrine system in hepatectomy rats and the involvement of arginine vasopressin (AVP) signaling in hypothalamus after EA was observed. Rats were randomly assigned to four groups, including the intact group, model group, sham‐EA group, and EA group. EA was given during the perioperative period at the Zusanli (ST36) and Sanyinjiao (SP6) points after hepatectomy. The serum adrenocorticotropic hormone (ACTH) and corticosterone (CORT) levels were detected via radioimmunoassay. The expression of AVP, arginine vasopressin receptor 1a (AVPR1a), arginine vasopressin receptor 1b (AVPR1b), and glucocorticoid receptor (GR) was detected by Western blot after surgery. Compared with the intact group, the ACTH and CORT levels in the serum of model group were increased, whereas the ACTH and CORT levels were decreased in the EA group compared with the model group. Moreover, AVP and AVPR1b protein levels in the pituitary gland were increased in the model group and decreased in the EA group. Further, a distinct increase in the AVP and AVPR1a protein levels was observed in the model group, whereas they were significantly decreased in the EA group. Blockade of AVPR1b by nelivaptan reduced the increase of ACTH and CORT. D [Leu4, Lys8] vasopressin can inhibit the effect of EA in rectification of the hyperactivity of the hypothalamic‐pituitary‐adrenal (HPA) axis. EA application at ST36 and SP6 can ameliorate the hyperactivity of the HPA axis via AVP signaling during the perioperative period.
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影响因子：
15.9
作者：
Tanoue, A;Ito, S;Tsujimoto, G
通讯作者：
Tsujimoto, G
影响因子：
82.9
作者：
通讯作者：
--
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影响因子：
3.6
作者：
Wang XW;Hu S;Mao-Ying QL;Li Q;Yang CJ;Zhang H;Mi WL;Wu GC;Wang YQ
通讯作者：
Wang YQ
影响因子：
--
作者：
LU, GW
通讯作者：
LU, GW
影响因子：
4
作者：
Eshkevari, Ladan;Permaul, Eva;Mulroney, Susan E.
通讯作者：
Mulroney, Susan E.