Long-term storage modifies the microRNA expression profile of cryopreserved human semen.
Long-term storage modifies the microRNA expression profile of cryopreserved human semen.
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DOI：
10.17305/bb.2023.9421
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发表时间：
2024-01-03
期刊：
影响因子：
--
通讯作者：
Zhu, Wen -Bing
中科院分区：
文献类型：
--
作者：
Huang, Chuan;Ji, Xi-Ren;Huang, Zeng-Hui;Liu, Qian;Wang, Rui-Jun;Fan, Li-Qing;Wu, Hui -Lan;Bo, Hao;Zhu, Wen -Bing
人类精液冷冻保存的全球实践在辅助生殖技术（ART）实验室和精子库中很常见。然而，长期冷冻保存对精液影响的信息仅限于临床数据总结和描述。在本研究中，我们制备了4份新鲜精液标本，4份冷冻保存至少1年的标本，3份冷冻保存至少5年的标本，4份冷冻保存至少10年的标本，3份冷冻保存至少15年的标本。从每个样品中提取总RNA，扩增，标记，并映射到miRBase数据库中已知的主要microRNA（miRNA），从而能够预测新的miRNA。我们发现低温保存可以导致miRNA表达的变化，并且随着保存时间的增加，这些变化变得更加明显。同时，let-7 d-3 p、let-7 c-5 p和let-7i-3 p miRNAs在冻融人精子中的表达随冻存时间动态变化。此外，我们分析了冻存表达的miRNAs及其靶mRNA的时间依赖性动力学，发现一半的靶基因在卵母细胞中表达。这些交叉基因主要富集在癌症和细胞骨架信号通路中。我们的研究结果表明，冷冻保存的人类精液的miRNA表达谱被长期储存所改变。此外，随着储存时间的增加，对人类精子的影响在miRNA方面变得更加明显，这可能会对随后的受精和胚胎发育产生影响。
The global practice of cryopreservation of human semen is commonplace in Assisted Reproductive Technology (ART) labs and sperm banks. However, information on the effects of long-term cryopreservation on semen is limited to clinical data summaries and descriptions. For this study, we prepared 4 semen specimens of fresh semen, 4 specimens cryostored for at least 1 year, 3 specimens cryostored for at least 5 years, 4 specimens cryostored for at least 10 years, and 3 specimens cryostored for at least 15 years. Total RNA was extracted from each sample, amplified, labeled, and mapped to the known primary microRNA (miRNA) in the miRBase database, enabling the prediction of novel miRNAs. We found that cryopreservation can lead to changes in miRNA expression, and with the increase in storage time, these changes became more pronounced. Meanwhile, the expression of let-7d-3p, let-7c-5p, and let-7i-3p miRNAs changed dynamically over cryostorage time in frozen-thawed human sperm. Furthermore, we analyzed the time-dependent dynamics of cryostorage-expressed miRNAs and their target mRNAs and found that half of the target genes were expressed in oocytes. These intersection genes were mainly enriched in cancer and cytoskeletal signaling pathways. Our findings showed that the miRNA expression profile of cryopreserved human semen is modified by long-term storage. Furthermore, as the storage time increases, the impact on human sperm becomes more pronounced in terms of miRNAs, which may have an effect on subsequent fertilization and embryonic development.
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DOI：
10.1242/dev.083279
发表时间：
2012-12
期刊：
Development (Cambridge, England)
影响因子：
--
作者：
Vogt EJ;Meglicki M;Hartung KI;Borsuk E;Behr R
通讯作者：
Behr R
影响因子：
3.7
作者：
Li J;Zhu L;Huang J;Liu W;Han W;Huang G
通讯作者：
Huang G
影响因子：
2.9
作者：
Huang C;Tang YL;Hu JL;Zhou WJ;Huang ZH;Luo XF;Li Z;Zhu WB
通讯作者：
Zhu WB
影响因子：
4.4
作者：
Kamijo, Shintaro;Hamatani, Toshio;Sasaki, Hiroyuki;Suzuki, Hiroki;Abe, Akane;Inoue, Osamu;Iwai, Maki;Ogawa, Seiji;Odawara, Kei;Tanaka, Kanako;Mikashima, Mutsumi;Suzuki, Masami;Miyado, Kenji;Matoba, Ryo;Odawara, Yasushi;Tanaka, Mamoru
通讯作者：
Tanaka, Mamoru
单亲胚胎的单细胞转录组分析揭示了亲本对人类植入前发育的影响
影响因子：
23.9
作者：
Leng, Lizhi;Sun, Jiya;Lin, Ge
通讯作者：
Lin, Ge